新兴证据显示，循环RNA在各种疾病，包括癌症的发展和进展中发挥着重要的生物学效应。我们的研究旨在分析hsa-circ_0003570在肝细胞癌（HCC）发展中的分子生物学效应。功能实验结果表明，circ_0003570的敲低可诱导HCC细胞生长、迁移和侵袭，而circ_0003570的过表达则呈现相反的效应。在体内实验中，来自circ_过表达细胞的异种移植瘤体积和重量比对照组小。进一步的研究表明，circ_0003570可能通过与miR-182-5p竞争性结合，调节下游靶基因STARD13的抑制，从而发挥作为竞争性内源RNA的功能，这些被双荧光素酶报告试验和功能恢复试验所证实。总之，circ_0003570通过调节miR-182-5p/STARD13通路抑制了HCC的发展。©2022年作者或许可方。

Emerging evidence have revealed that circRNAs exert important biological effects in the development and progression of various diseases, including cancer. Our study aimed to elaborated the biological effects of hsa-circ_0003570 in hepatocellular carcinoma (HCC) development at the molecular level.The results of functional experiments showed that knockdown of circ_0003570 induced HCC cell growth, migration and invasion, whereas overexpression of circ_0003570 presented the opposite effects. In vivo experiments, xenograft tumors grown from circ-overexpressed cells had smaller tumor volume and weight than the control group. Further investigations suggested that circ_0003570 may function as a competing endogenous RNA via competitively binding miR-182-5p and thereby regulating the repression of downstream target gene STARD13, which were demonstrated by dual luciferase reporter assay and functional rescued experiments.Taken together, circ_0003570 suppresses the development of HCC by modulating miR-182-5p/STARD13 axis.© 2022. The Author(s).