随着骨骼肌组织工程的进步，新的平台涌现，具有重要的生物学研究、疾病模拟和药物测试应用。当前的发展突出了工程骨骼肌组织的更高复杂性的追求。这些新的人类骨骼肌组织模型将成为药物发现和开发以及疾病模拟的强大工具。作者回顾了用于测试药物的工程骨骼肌组织体外模型的最新进展，重点关注四种主要的细胞培养技术的应用：孔板细胞培养、微流控、器官样和生物打印构造。此外，还提供了有关卫星细胞微环境的附加信息。近年来，越来越复杂的骨骼肌组织体外模型已经制造出来。在干细胞研究和人类骨骼肌组织工程方面已经取得了重要进展。一些平台已经开始用于药物测试，尤其是基于肥大/萎缩参数和肌纤维收缩能力的平台。通过使用多细胞类型和多材料作为基质，预计将有更多的发展。现在应该增加对这些模型在药物测试中的验证和使用。

With the advances in skeletal muscle tissue engineering, new platforms have arisen with important applications in biology studies, disease modeling, and drug testing. Current developments highlight the quest for engineering skeletal muscle tissues with higher complexity . These new human skeletal muscle tissue models will be powerful tools for drug discovery and development and disease modeling.The authors review the latest advances in in vitro models of engineered skeletal muscle tissues used for testing drugs with a focus on the use of four main cell culture techniques: Cell cultures in well plates, in microfluidics, in organoids, and in bioprinted constructs. Additional information is provided on the satellite cell niche.In recent years, more sophisticated in vitro models of skeletal muscle tissues have been fabricated. Important developments have been made in stem cell research and in the engineering of human skeletal muscle tissue. Some platforms have already started to be used for drug testing, notably those based on the parameters of hypertrophy/atrophy and the contractibility of myotubes. More developments are expected through the use of multicellular types and multi-materials as matrices . The validation and use of these models in drug testing should now increase.