硫化氢（H2S）作为一个关键的基础气体传递物质，调节着各种生物过程，而无法控制的H2S与多种疾病的发生和发展密不可分，包括癌症。作为一种侵入性肿瘤治疗技术，光动力疗法（PDT）也受到了极大的关注。由于升高的H2S在癌症中的关键作用，将H2S耗竭/识别和PDT结合起来应该是一种有效的增强抗癌性能的策略。在这项工作中，我们通过3RAX和NBD的化学连接报告了一种H2S降解辅助PDT平台（3RAX-NBD）。3RAX-NBD能够快速与H2S反应，并在体内外产生一种新的3RAX衍生化合物3，其荧光增强。更值得注意的是，3RAX-NBD能够通过原位照射有效地杀死多种癌细胞，并且3RAX-NBD在4T1肿瘤携带BALB/c雌性小鼠上也具有显著的抗癌作用，而且没有明显的毒副作用。我们相信，我们的H2S降解辅助PDT平台可能为疾病中H2S的关键作用提供了一个强大的工具，同时也为癌症治疗提供了另一种有前途的候选方案。版权所有 © 2022. Elsevier B.V.发表。

Hydrogen sulfide (H2S) as a key fundamental gasotransmitter regulates various biological processes, and the incontrollable H2S is essentially associated with the occurrence and development of multiple diseases, including cancers. Photodynamic therapy (PDT), as an invasive tumor treatment technology, has also attracted great attentions. Due to the key role of elevated H2S in cancers, integrating H2S depletion/recognition and PDT should be an effective strategy to enhance anticancer performance. In this work, we report a H2S depletion aided PDT platform (3RAX-NBD) by the chemical ligation of 3RAX and NBD. 3RAX-NBD can react rapidly with H2S and generate a novel 3RAX derivative compound 3 with increased fluorescence in vitro and in vivo. More notably, 3RAX-NBD can effectively kill multiple cancer cells through in situ irradiation, and 3RAX-NBD also has prominent anticancer effects on 4 T1 tumor-bearing BALB/c female mice with no notably toxic side effects. We believe that our H2S depletion aided PDT platform may provide a powerful tool for studying the key roles of H2S in diseases, and also give another promising candidate for cancer treatment.Copyright © 2022. Published by Elsevier B.V.