磁性纳米粒子在医疗应用中得到越来越广泛的应用，包括通过磁致热疗法治疗癌症。本协议描述了一种溶剂热基础下的过程，制备克级别的铁氧体纳米粒子，其形状明确定义为纳米立方体、纳米星星和其他多面体纳米粒子，并对结构/磁性属性进行精细控制，以实现参考点的磁致热疗法性能。这种简单的方法包括简单的步骤：(i)制备表面活性剂和脂肪胺的均匀酒精溶液；(ii)加入有机金属前体和作为关键形状指导剂的醛分子；(iii)在高压釜中进行溶剂热结晶反应。铁氧体纳米粒子的形状可以通过醛基配体的结构进行控制。苯甲醛及其芳香族衍生物有利于形成立方体铁氧体纳米粒子，而脂肪族醛则导致形成球形纳米粒子。将用于纳米立方体合成的一级胺替换为二级/三级胺，则产生具有星状形状的纳米粒子。在形状、窄的大小分布（低于5%）、组成调控和晶体性方面的良好掌控保证了制备克级别的纳米立方体/星状纳米粒子，在临床使用的磁场条件下，具有与通过热分解法典型制备的毫克级别相当甚至更高的特定吸附速率。在大约10小时内，具平均化学水平的人可制备出基准磁致热疗法应用的克级别纳米粒子产品。©2023 Springer Nature Limited.

Magnetic nanoparticles are increasingly used in medical applications, including cancer treatment by magnetic hyperthermia. This protocol describes a solvothermal-based process to prepare, at the gram scale, ferrite nanoparticles with well-defined shape, i.e., nanocubes, nanostars and other faceted nanoparticles, and with fine control of structural/magnetic properties to achieve point-of-reference magnetic hyperthermia performance. This straightforward method comprises simple steps: (i) making a homogeneous alcoholic solution of a surfactant and an alkyl amine; (ii) adding an organometallic metal precursor together with an aldehyde molecule, which acts as the key shape directing agent; and (iii) reacting the mixture in an autoclave for solvothermal crystallization. The shape of the ferrite nanoparticles can be controlled by the structure of the aldehyde ligand. Benzaldehyde and its aromatic derivatives favor the formation of cubic ferrite nanoparticles while aliphatic aldehydes result in spherical nanoparticles. The replacement of the primary amine, used in the nanocubes synthesis, with a secondary/tertiary amine results in nanoparticles with star-like shape. The well-defined control in terms of shape, narrow size distribution (below 5%), compositional tuning and crystallinity guarantees the preparation, at the gram scale, of nanocubes/star-like nanoparticles that possess, under magnetic field conditions of clinical use, specific adsorption rates comparable to or even superior to those obtained through thermal decomposition methods, which are typically prepared at the milligram scale. Here, gram-scale nanoparticle products with benchmark features for magnetic hyperthermia applications can be prepared in ~10 h with an average level of expertise in chemistry.© 2023. Springer Nature Limited.