細胞激素分泌的B細胞在改變慢性炎症性疾病的免疫反應中扮演了重要的角色。我們對患有牙周炎的患者中與牙周炎相關的細菌進行了B細胞細胞激素反應的特徵分析。我們從牙周炎B級（N = 31）或C級（N = 25）的患者和25名健康對照組（HCs）收集了血液和唾液樣本。單核細胞被刺激以Porphyromonas gingivalis，Fusobacterium nucleatum，Staphylococcus epidermidis或Cutibacterium acnes，並通過流式細胞儀評估B細胞產生的腫瘤壞死因子（TNF）-α，白細胞介素（IL）-6，干擾素（IFN）-γ，IL-10和轉化生長因子（TGF）-β的細胞激素。與B級患者相比，HCs具有更高的基線頻率的B細胞產生IFN-γ或TNF-α，但只有B細胞來自B級患者，對P. gingivalis和F. nucleatum的挑戰后，才表現出顯著的分化，進入IFN-γ，TNF-α，TGF-β或IL-10產生細胞。值得注意的是，C級患者的IL-10產生B細胞的基線頻率與臨床附著損失呈反比例關係。大部分IFN-γ和TGF-β產生的B細胞是CD27 +記憶細胞，而產生IL-10的B細胞主要是CD27-CD5-。來自B級患者的B細胞，特別是那些携带P. gingivalis的B細胞，對P. gingivalis表現出促炎性的B細胞反應。此外，C級組中的IL-10產生B細胞的基線頻率與AL的反比例關係相關，表明IL-10產生B細胞的免疫調節能力降低。此文件受版權保護。保留所有權利。

Cytokine-producing B cells play a well-established role in modifying immune responses in chronic inflammatory diseases. We characterized B-cell cytokine responses against periodontitis-associated bacteria in patients with periodontitis.Blood and saliva samples were collected from patients with periodontitis grade B (N = 31) or grade C (N = 25), and 25 healthy controls (HCs). Mononuclear cells were stimulated with Porphyromonas gingivalis, Fusobacterium nucleatum, Staphylococcus epidermidis or Cutibacterium acnes, and B-cell production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, interferon (IFN)-γ, IL-10 and transforming growth factor (TGF)-β by B cells was assessed by flow cytometry.HCs had higher baseline frequencies of B cells producing IFN-γ or TNF-α than grade B patients, but only B cells from grade B patients showed significant differentiation into IFN-γ-, TNF-α-, TGF-β- or IL-10-producing cells after challenge with P. gingivalis and into IFN-γ-, TGF-β- or IL-10-producing cells after challenge F. nucleatum. Notably, the baseline frequency of IL-10-producing B cells from grade C patients correlated inversely with clinical attachment loss. The major proportion of the IFN-γ- and TGF-β-producing B cells were CD27+ memory cells, while the IL-10-producing B cells were mainly CD27-CD5-.B cells from grade B patients, particularly those harboring P. gingivalis, showed pro-inflammatory B-cell responses to P. gingivalis. Moreover, the baseline frequency of IL-10-producing B cells in the grade C group correlated inversely with AL, suggesting a diminished immunoregulatory capacity of IL-10-producing B cells. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.