高对比度和高分辨率成像技术能够实时敏感地检测胃肠道病变。此研究旨在探讨使用莫西沙星和前荧光素的新型双荧光成像在检测人类胃肠道肿瘤中的可行性。前瞻性招募了患有结肠或胃肿瘤的患者。使用镊子或内窥镜切除病变组织。在局部滴入莫西沙星和前荧光素后，使用定制的轴向扫描广域荧光显微镜进行双荧光成像。成像结果与细胞标记的共聚焦成像和传统组织学检查进行比较。评估了来自八名患者的十个结肠样本（一个正常粘膜，九个腺瘤）和来自四名患者的六个胃样本（一个正常粘膜，五个腺瘤）。双荧光成像显示了详细的细胞结构。在正常粘膜中观察到规律的腺体结构和极化的细胞排列。在正常结肠粘膜中保留了黏液细胞。在腺瘤中观察到不规则的腺状结构，细胞浆稀少，核呈分散状伸长。结肠病变中黏液细胞稀少或丧失。莫西沙星和前荧光素成像之间的相似性分析显示，在腺瘤中相对于正常粘膜，相关系数相对较高。双荧光成像在结肠和胃病变中显示了良好的检测准确率（分别为82.3％和86.0％）。高对比度和高分辨率双荧光成像可用于在胃肠道肿瘤中获取详细的组织病理信息。需要进一步研究双荧光成像作为体内实时视觉诊断方法的发展。© 2023 Wiley Periodicals LLC.

High-contrast and high-resolution imaging techniques would enable real-time sensitive detection of the gastrointestinal lesions. This study aimed to investigate the feasibility of novel dual fluorescence imaging using moxifloxacin and proflavine in the detection of neoplastic lesions of the human gastrointestinal tract.Patients with the colonic and gastric neoplastic lesions were prospectively enrolled. The lesions were biopsied with forceps or endoscopically resected. Dual fluorescence imaging was performed by using custom axially swept wide-field fluorescence microscopy after topical moxifloxacin and proflavine instillation. Imaging results were compared with both confocal imaging with cell labeling and conventional histological examination.Ten colonic samples (one normal mucosa, nine adenomas) from eight patients and six gastric samples (one normal mucosa, five adenomas) from four patients were evaluated. Dual fluorescence imaging visualized detail cellular structures. Regular glandular structures with polarized cell arrangement were observed in normal mucosa. Goblet cells were preserved in normal colonic mucosa. Irregular glandular structures with scanty cytoplasm and dispersed elongated nuclei were observed in adenomas. Goblet cells were scarce or lost in the colonic lesions. Similarity analysis between moxifloxacin and proflavine imaging showed relatively high correlation values in adenoma compared with those in normal mucosa. Dual fluorescence imaging showed good detection accuracies of 82.3% and 86.0% in the colonic and the gastric lesions, respectively.High-contrast and high-resolution dual fluorescence imaging was feasible for obtaining detail histopathological information in the gastrointestinal neoplastic lesions. Further studies are needed to develop dual fluorescence imaging as an in vivo real-time visual diagnostic method.© 2023 Wiley Periodicals LLC.