毛发孢病对应于一种系统真菌病，导致高死亡率，并且经常与医疗设备有关。它主要影响免疫抑制患者，并且与获得性人类免疫缺陷、器官和组织移植以及白血病和淋巴瘤等恶性血液病密切相关。全球范围内越来越多的毛发孢感染病例被报道，然而，目前对其特征或致病微生物的了解仍然很少。因此，本研究的目的是：通过验证分离株的生物膜形成能力，研究毛发孢属59株酵母菌的情况；通过将欧洲抗微生物敏感性测试委员会（EUCAST）微量化学稀释法与商业法Etest进行比较，分析游离细胞对抗真菌药物氟康唑，伊曲康唑，两性霉素B，伏立康唑和卡泊芬净的敏感性模式；并通过微量化学稀释法评估生物膜细胞（固定）对同一抗真菌药物的敏感性模式。所有分离物均在聚苯乙烯板表面形成了生物膜，54.3％的样本被认为是强生产者。抗真菌敏感性技术的比较表明，Etest显示的MICs比微量稀释法得到的MICs更高且不一致，特别是在氟康唑，伊曲康唑和卡泊芬净方面。考虑到生物膜的敏感性，大多数品种对测试的抗真菌药物的MIC50和MIC90很高，显示出两性霉素B的浓度比原始值高4到66倍，卡泊芬净的浓度比原始值高2到33倍。这些结果强调了进一步对毛发孢属进行研究的重要性，以便比较实验室发现和体内反应，同时考虑生物膜细胞对药物的敏感性测试和行为。©2023年，作者以牛津大学出版社的名义发表，代表人类和动物真菌学国际学会。

Trichosporonosis corresponds to a systemic fungal disease that leads to high mortality rates and is frequently associated with medical devices. It affects immunosuppressed patients in particular and is strongly linked to acquired human immunodeficiency, organ and tissue transplants and malignant hematologic diseases such as leukemia and lymphomas. Trichosporon infections have been increasingly reported worldwide; however, little information is available either about their characteristics or about the causative microorganism. Thus, the aims of the present study were: to investigate 59 yeasts of the genus Trichosporon by verifying the biofilm formation capacity of isolates; to analyze the susceptibility patterns of planktonic cells against the antifungals fluconazole, itraconazole, amphotericin-B, voriconazole and caspofungin by comparing European Committee for Antimicrobial Susceptibility Testing (EUCAST) broth microdilution technique with the commercial method Etest, and to assess the susceptibility patterns of biofilm cells (sessile) against the same antifungals through broth microdilution. The ability to form biofilm on the surface of polystyrene plates was noted for all isolates, and 54.3% samples were considered strong producers. Comparison between the antifungal susceptibility techniques evidenced that Etest showed higher and discordant MICs from those obtained by the microdilution method, especially for fluconazole, itraconazole and caspofungin. Considering the susceptibility of biofilms, most species had high MIC50 and MIC90 against the tested antifungals, showing 4-to-66-fold higher concentrations for amphotericin B and 2-to-33-fold greater concentrations for caspofungin. These results highlight the importance of further studies with Trichosporon spp. for comparison between laboratory findings and in vivo response, considering both the susceptibility tests and the behavior of biofilm cells against drugs.© The Author(s) 2023. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.