传统的癌症治疗，如化疗，通常作为单一治疗方案时难以实现完全疗效，因此，目前的治疗策略通常依赖于多种方法的组合。纳米颗粒光热疗法（PTT）是一种通过局部、严重的高温来杀死癌细胞的技术，已被证明是多种癌症治疗的一种有前途的辅助治疗方法。在这里，我们评估了基于金纳米壳（NS）的PTT和脂质体阿霉素联合应用是否可以提高结直肠癌小鼠模型的治疗效果。首先，在肿瘤携带的小鼠上进行NS基于PTT的实验。然后注射放射性标记的脂质体，以确定在PTT后的最佳注射时间和观察脂质体在肿瘤中的分布情况。最后，我们结合PTT和阿霉素负载脂质体，并通过跟踪肿瘤生长和生存来研究治疗策略的效果。PTT显著提高了脂质体在肿瘤内的积累，但仅当脂质体在疗法后立即注射时才能实现。脂质体主要积累在激光消融区附近的区域。当PTT与阿霉素负载脂质体结合时，小鼠的肿瘤生长减缓，生存得到改善。根据我们的临床前研究结果，基于NS的PTT似乎有望成为脂质体化疗和其他系统治疗的辅助治疗方法，并在临床应用中具有潜在的相关性。 © 2023 Simón等

Traditional cancer treatments, such as chemotherapy, are often incapable of achieving complete responses as standalone therapies. Hence, current treatment strategies typically rely on a combination of several approaches. Nanoparticle-based photothermal therapy (PTT) is a technique used to kill cancer cells through localized, severe hyperthermia that has shown promise as an add-on treatment to multiple cancer therapies. Here, we evaluated whether the combination of gold nanoshell (NS)-based PTT and liposomal doxorubicin could improve outcome in a mouse model of colorectal cancer.First, NS-based PTT was performed on tumor-bearing mice. Radiolabeled liposomes were then injected at different timepoints to follow their accumulation in the tumor and determine the ideal injection time after PTT. In addition, fluorescent liposomes were used to observe the liposomal distribution in the tumor after PTT. Finally, we combined PTT and doxorubicin-loaded liposomes and studied the effect of the treatment strategy on the mice by following tumor growth and survival.PTT significantly improved liposomal accumulation in the tumor, but only when the liposomes were injected immediately after the therapy. The liposomes accumulated mostly in regions adjacent to the ablated areas. When PTT was combined with liposomal doxorubicin, the mice experienced a slowdown in tumor growth and an improvement in survival.According to our preclinical study, NS-based PTT seems promising as an add-on treatment for liposomal chemotherapy and potentially other systemic therapies, and could be relevant for future application in a clinical setting.© 2023 Simón et al.