子宫内膜癌（EC）是女性高发的一种恶性肿瘤，高危患者疾病进展后的生存率显著降低。长非编码RNA（LncRNA）在肿瘤中的调节作用已得到广泛认可，但在EC中的研究尚少。为了研究HOXB-AS3在EC中的影响，我们利用生物信息学工具进行预测，并收集临床样本检测HOXB-AS3的表达。使用集落形成实验、MTT实验、细胞流式仪和凋亡实验，以及穿透实验验证HOXB-AS3在EC中的作用。HOXB-AS3在EC中上调，促进EC细胞的增殖和侵袭能力，并抑制凋亡。此外，ROC曲线说明其诊断价值。我们通过慢病毒转导、FISH、Oil Red O染色、TC和FFA含量检测、RNA-pulldown、RIP等机制探索实验，揭示HOXB-AS3可以结合PTBP1并共同调节SREBP1的表达，从而调节EC细胞的脂质代谢。据我们所知，这是关于EC中脂质代谢紊乱的HOXB-AS3的首个研究。此外，我们认为HOXB-AS3有成为肿瘤标记物或治疗靶点的潜力。版权所有 © 2023 Elsevier Inc. 发布。

Endometrial cancer (EC) is a malignant tumor with a high incidence in women, and the survival rate of high-risk patients decreases significantly after disease progression. The regulatory role of long non-coding RNAs (LncRNAs) in tumors has been widely appreciated, but there have been few studies in EC. To investigate the effect of HOXB-AS3 in EC, we used bioinformatics tools for prediction and collected clinical samples to detect the expression of HOXB-AS3. Colony formation assay, MTT assay, flow cytometry and apoptosis assay, and transwell assay were used to verify the role of HOXB-AS3 in EC. HOXB-AS3 was upregulated in EC, promoted the proliferation and invasive ability of EC cells, and inhibited apoptosis. In addition, the ROC curve illustrated its diagnostic value. We explored experiments via lentiviral transduction, FISH, Oil Red O staining, TC and FFA content detection, RNA-pulldown, RIP, and other mechanisms to reveal that HOXB-AS3 can bind to PTBP1 and co-regulate the expression of SREBP1, thereby regulating lipid metabolism in EC cells. To the best of our knowledge, this is the first study on HOXB-AS3 in disorders of lipid metabolism in EC. In addition, we believe HOXB-AS3 has the potential to be a neoplastic marker or a therapeutic target.Copyright © 2023. Published by Elsevier Inc.