吻合术是一种标准技术，可用于不同情况的梗阻、肿瘤和创伤。梗阻、粘连或吻合处漏出是可能的并发症。为了提高愈合效果和预防术后并发症，我们设计了一种混合支架，其中包含无细胞人类羊膜膜和聚己内酯-二硫化钼纳米片，用于结肠吻合术。在两组大鼠（对照组和支架组）上进行了结肠-结肠吻合模型实验。支架组将混合支架包裹在吻合处。于术后第三天和第七天切除吻合处的样本，进行组织病理和分子评估。支架组的组织病理评分和破裂压力明显改善。支架组没有发生死亡和吻合处漏出。此外，炎症标志物明显降低，反炎细胞因子在支架组中增加。结果表明，我们的混合支架是结直肠吻合术修复的合适选择，可以减少术后并发症并加速愈合。©2023年作者，独家授权给Springer-Verlag GmbH Germany一部分Springer Nature。

Anastomosis is a standard technique following different conditions such as obstruction, tumor, and trauma. Obstruction, adhesion, or anastomosis leakage can be some of its complications. To improve healing and prevent postoperative complications, we design a hybrid scaffold containing acellular human amniotic membranes and polycaprolactone-molybdenum disulfide nanosheets for colon anastomosis. The animal model of colocolonic anastomosis was performed on two groups of rats: control and scaffold. The hybrid scaffold was warped around the anastomosis site in the scaffold group. Samples from the anastomosis site were resected on the third and seventh postoperative days for histopathological and molecular assessments. Histopathologic score and burst pressure had shown significant improvement in the scaffold group. No mortality and anastomosis leakage was reported in the scaffold group. In addition, inflammatory markers were significantly decreased, while anti-inflammatory cytokines were increased in the scaffold group. The result indicates that our hybrid scaffold is a proper choice for colorectal anastomosis repair by declining postoperative complications and accelerating healing.© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.