幽门螺杆菌调节宿主的炎症反应，导致慢性胃炎，从而促进胃癌的发病。我们通过抑制幽门螺杆菌引起的炎症活性来验证三叶木通的对幽门螺杆菌感染的影响。给予5周龄的C57BL/6小鼠（n = 8）三叶木通叶提取物（10或20 mg / kg每天）6周。进行侵入性检测（类似幽门曲菌的微生物[CLO]）和非侵入性检测（粪便抗原测试[SAT]和幽门螺杆菌抗体酶联免疫吸附测定）以确认幽门螺杆菌的根除。为评估三叶木通的抗炎作用，测量老鼠胃组织的促炎症细胞因子水平和炎症评分。三叶木通显著降低了CLO得分和幽门螺杆菌免疫球蛋白G抗体的光密度水平，在每天10和20 mg / kg的剂量下（P <0.05）。三叶木通以浓度依赖性方式降低了幽门螺杆菌抗体水平，将SAT的阴性反应增加了37.5％，并抑制了促炎症细胞因子白细胞介素（IL; IL-1β，IL-6，1L-8和肿瘤坏死因子α）。三叶木通还缓解了胃侵蚀和溃疡，并显著降低了炎症评分（P <0.05）。我们以芦丁作为高效液相色谱的标准来测量三叶木通提取物中的芦丁含量。三叶木通叶提取物通过抑制炎症表现出抗幽门螺杆菌活性。我们的发现表明，三叶木通叶提取物可能是一种有效的幽门螺杆菌抗菌剂。

Helicobacter pylori modulates the host inflammatory response, resulting in chronic gastritis, which contributes to gastric cancer pathogenesis. We verified the effect of Cudrania tricuspidata on H. pylori infection by inhibiting H. pylori-induced inflammatory activity. Five-week-old C57BL/6 mice (n = 8) were administered C. tricuspidata leaf extract (10 or 20 mg/kg per day) for 6 weeks. An invasive test (campylobacter-like organism [CLO]) and noninvasive tests (stool antigen test [SAT] and H. pylori antibody enzyme-linked immunosorbent assay) were performed to confirm the eradication of H. pylori. To evaluate the anti-inflammatory effect of C. tricuspidata, pro-inflammatory cytokines levels and inflammation scores were measured in mouse gastric tissue. C. tricuspidata significantly decreased the CLO score and H. pylori immunoglobulin G antibody optical density levels at both 10 and 20 mg/kg per day doses (P < .05). C. tricuspidata decreased the H. pylori antibody levels in a concentration-dependent manner, increased negative responses to SAT by up to 37.5%, and inhibited the pro-inflammatory cytokines interleukin (IL; IL-1β, IL-6, 1L-8, and tumor necrosis factor alpha). C. tricuspidata also relieved gastric erosions and ulcers and significantly reduced the inflammation score (P < .05). We measured rutin in C. tricuspidata extract as a standard for high-performance liquid chromatography. C. tricuspidata leaf extract showed anti-H. pylori activity through the inhibition of inflammation. Our findings suggest that C. tricuspidata leaf extract is potentially an effective functional food material against H. pylori.