Sjögren综合征（SjS）是一种免疫性疾病，其特点是破坏外分泌腺上皮细胞，导致粘膜干燥症状，主要的危及生命的并发症是淋巴瘤。在这种疾病中需要新的生物标志物，尤其是对于那些患有真正干燥症状但不符合当前标准的患者的诊断生物标志物，以及对于淋巴瘤风险患者的预后生物标志物。质膜外囊泡（EVs）在几种疾病中是有希望的生物标志物候选物，但其在SjS中的潜力尚未得到探索。在这个概念验证研究中，我们对原发性SjS患者（pSS，n=12）和健康供体（HD，n=8）以及系统性红斑狼疮患者（SLE，n=12）的血浆EVs进行了表型和蛋白质组学水平的特征化。我们发现特定的血浆EVs亚群，来自中性粒细胞、内皮细胞和上皮细胞，pSS中增加。我们还在血浆EVs中鉴定出一个pSS蛋白质组学标志，包括中性粒细胞、上皮细胞和内皮细胞相关的蛋白质，例如αM整合素（ITGAM）、olfactomedin-4（OLFM4）、Ras相关蛋白RAB10和CD36。总的来说，我们的结果支持血浆EVs作为SjS生物标志物的相关性。版权所有 © 2023 Ferrant、Pontis、Zimmermann、Dingli、Poullet、Loew、Tarte和Dumontet。

Sjögren syndrome (SjS) is an autoimmune disease characterized by the destruction of the exocrine gland epithelia, causing a dryness of mucosa called sicca symptoms, and whose main life-threatening complication is lymphoma. There is a need for new biomarkers in this disease, notably diagnostic biomarkers for patients with genuine sicca symptoms that do not meet current criteria, and prognostic biomarkers for patients at risk of lymphoma. Plasma extracellular vesicles (EVs) are promising biomarker candidates in several diseases, but their potential has not yet been explored in SjS. In this proof-of-concept study, we characterized EVs from primary SjS patients (pSS, n=12) at the phenotypic and proteomic levels, compared to EVs from healthy donor (HD, n=8) and systemic lupus erythematosus patients (SLE, n=12). Specific plasma EVs subpopulations, derived from neutrophils, endothelial, and epithelial cells, were found increased in pSS. We also identified a pSS proteomic signature in plasma EVs, including neutrophil-, epithelial-, and endothelial-related proteins, such as integrin alpha M (ITGAM), olfactomedin-4 (OLFM4), Ras-related protein RAB10, and CD36. Overall, our results support the relevance of plasma EVs as biomarkers in SjS.Copyright © 2023 Ferrant, Pontis, Zimmermann, Dingli, Poullet, Loew, Tarte and Dumontet.