背景 结直肠癌（CRC）是导致癌症相关死亡的第二大原因。尽管存在手术、化疗、放疗和免疫疗法等综合治疗方法，但预后仍然不佳，主要是因为复发和远处转移。癌干细胞（CSC）被认为是肿瘤的发展和扩散的原因。因此，针对这些细胞的靶向治疗希望减少复发和转移的机会，改善预后。已经确定了许多免疫标记物以检测CRC中的CSC。在这里，我们试图评估CD133干细胞标记物在结直肠癌组织中的免疫组织化学表达，并与各种病理学参数进行相关性分析。 方法共分析了51例CRC病例。在实验室标准化后进行CD133的免疫组织化学检测。根据乘以强度评分和百分比评分所得到的总分，确定表达状态。将CD133的表达与患者的年龄、性别、肿瘤部位、组织学分级、侵袭程度、淋巴管侵袭（LVI）、神经侵袭（PNI）和淋巴结状态进行相关性分析。 结果21例（41.17％）病例中显示高CD133表达。除肿瘤部位外，CD133表达与病理学参数之间没有显著关联。随着从近端结肠向直肠移动，CD133的表达显著增加。 结论与近段结肠相比，CD133在大肠的远段部位显著增高。但是，CD133的表达与组织学分级、侵袭程度或淋巴结状态之间并没有线性相关性。版权所有 © 2023，Suman等人。

Background Colorectal carcinoma (CRC) is the second-leading cause of cancer-related death. Despite the combined (surgery, chemotherapy, radiotherapy, and immunotherapy) modalities of treatment, the prognosis remains poor, mostly because of recurrence and distant metastasis. Cancer stem cells (CSC) are thought to be responsible for the development and spread of tumors. Hence, targeted therapy against these cells hopes to reduce the chance of recurrence and metastasis and improve the prognosis. Many immune markers have been identified to detect CSC in CRC. Here, we tried to assess the immunohistochemical expression of the stem cell marker CD133 in colorectal carcinoma and its correlation with various pathological parameters. Methodology A total of 51 cases of CRC were analyzed. Immunohistochemistry for CD133 was done after standardization in our laboratory. Expression status was decided based on the total score obtained by multiplying the intensity score by the percentage score. CD133 expression was correlated with the age and gender of the patient, tumor location, histological grade, extent of invasion, lymphovascular invasion (LVI), perineural invasion (PNI), and nodal status. Results High CD133 expression was seen in 21 (41.17%) cases. There was no significant association between CD133 expression and the pathological parameters except the tumor site. CD133 expression was significantly higher as we moved from the proximal colon to the rectum. Conclusions CD133 expression was significantly higher in the distal part of the large intestine as compared to the proximal part. But there was no linear correlation between CD133 expression and histological grade, extent of invasion, or nodal status.Copyright © 2023, Suman et al.