甲状腺癌是一种常见的内分泌癌，其全球发病率一直在稳步增加。MEX3A是一种已知在多种人类恶性肿瘤中高表达的蛋白质，包括甲状腺癌，并且与患者预后有关。然而，MEX3A在甲状腺癌中的肿瘤形成能力的分子机制尚不完全了解。在本研究中，我们旨在研究MEX3A在甲状腺癌中的作用。我们确认MEX3A在甲状腺癌组织和细胞系中过度表达。此外，我们发现MEX3A的高水平与AJCC分期呈正相关。为了进一步了解MEX3A在甲状腺癌中的功能意义，我们抑制了B-CPAP和TPC-1细胞中的MEX3A表达。有趣的是，我们观察到MEX3A抑制可显著减少甲状腺癌细胞的增殖和迁移，改善细胞凋亡并阻止肿瘤生长。这些发现强烈表明MEX3A在甲状腺癌的发展中起着关键作用。此外，我们的研究揭示了MEX3A与CREB1（cAMP反应元件结合蛋白1）之间的重要相互作用。MEX3A与CREB1之间的相互作用似乎通过直接靶向CREB1来促进甲状腺癌的肿瘤发展。沉默CREB1观察到可以缓解MEX3A在甲状腺癌细胞中促进的恶性表型。综上所述，本研究突出了MEX3A-CREB1相互作用在甲状腺癌发展中的重要性，并提出了针对MEX3A治疗该疾病的治疗潜力。 © 2023 作者。细胞生物学国际由John Wiley & Sons Ltd代表国际细胞生物学联合会出版。

Thyroid cancer is a prevalent form of endocrine cancer, and its global incidence has been steadily increasing. MEX3A is a protein that is known to be highly expressed in various human malignant tumors, including thyroid cancer, and it has been linked to patient prognosis. However, the molecular mechanisms underlying MEX3A's tumorigenic capabilities in thyroid cancer are not fully understood. In this study, we aimed to investigate the role of MEX3A in thyroid cancer. We confirmed that MEX3A was overexpressed in both thyroid cancer tissues and cell lines. Additionally, we found a positive correlation between high levels of MEX3A and the AJCC stage. To further understand the functional significance of MEX3A in thyroid cancer, we depleted MEX3A expression in B-CPAP and TPC-1 cells. Interestingly, we observed a significant reduction in thyroid cancer cell proliferation and migration, as well as ameliorated cell apoptosis and arrested tumor growth upon MEX3A depletion. These findings strongly suggested that MEX3A played a critical role in the development of thyroid cancer. Furthermore, our study uncovered an important interaction between MEX3A and CREB1 (cAMP response element-binding protein 1). The interaction between MEX3A and CREB1 appeared to contribute to the tumor-promoting effects of MEX3A in thyroid cancer by directly targeting CREB1. Silencing CREB1 was observed to alleviate the malignant phenotypes promoted by MEX3A in thyroid cancer cells. Together, this study highlighted the importance of the MEX3A-CREB1 interaction in thyroid cancer development and suggested the therapeutic potential of targeting MEX3A for the treatment of this disease.© 2023 The Authors. Cell Biology International published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology.