米勒植物（学名：Pereskia aculeata Miller），是仙人掌科植物家族的成员，由于其含有多种具有生物活性的化合物，具有抗炎、抗癌和镇痛等多种生物活性，具有药理潜力。本研究评估了米勒植物醇提物（EEPA）的抗炎效应及其作用机制。在体外实验中，EEPA抑制了大鼠RAW264.7巨噬细胞中lipopolysaccharide（脂多糖）刺激后炎症因子NO、IL-6和PGE2的分泌（ P <0.05）；同时，EEPA处理可显著降低P-P38和P-MK2的水平，同时上调TTP的表达（P <0.05）。在体内抗炎活性实验中，EEPA可以显著减轻佐剂诱导性关节炎大鼠的足和关节肿胀程度，脾指数和TNF-α、IL-6血清浓度（P <0.05）。同样，EEPA处理可以抑制醋酸诱导的腹腔毛细血管Evans蓝染料渗出，并显著延长热刺激引起的疼痛反应时间（P <0.05）。综上所述，这些结果表明EEPA在体内外均具有抗炎作用。因此，该研究为基于米勒植物的新型抗炎治疗的开发提供了实验和技术支持。

Pereskia aculeata Miller, a member of the Cactaceae family, is a plant with pharmacological potential due to its containing compounds with various biological activities, which include anti-inflammatory, anti-cancer and analgesic activities. In this study, we evaluated the anti-inflammatory effects of an ethanolic extract of P. aculeata Miller (EEPA) and the signalling pathways by which it exerts these effects. In vitro, EEPA inhibited the secretion of inflammatory factors NO, IL-6 and PGE2 in ipopolysaccharide-stimulated RAW264.7 macrophages (P<0.05). Treatment of RAW264.7 cells with EEPA also significantly decreased the levels of P-P38 and P-MK2, while upregulating the expression of TTP (P<0.05). In vivo anti-inflammatory activity assays revealed that EEPA reduced the degree of foot and joint swelling, the splenic index and the serum concentrations of TNF-α and IL-6 in in adjuvant-induced arthritis rats (P<0.05). Similarly, EEPA treatment of mice inhibited the acetic acid-induced exudation of Evans blue dye from peritoneal capillaries and significantly prolonged heat-stimulated pain response time (P<0.05). Taken together, these results suggest that EEPA exerts anti-inflammatory effects in vitro and in vivo. Thus, this study provides experimental and technical support for the development of a novel anti-inflammatory treatment based on P. aculeata Miller.