海藻作为宝贵的天然酚类化合物（PCs）来源，具有抗氧化、抗炎性和潜在抗癌效应等各种健康益处。高效提取海藻中的酚类化合物对于发挥其进一步应用至关重要。本研究比较了不同溶剂（乙醇、甲醇、水、丙酮和乙酸乙酯）对四种海藻（Ascophyllum sp.、Fucus sp.、Ecklonia sp.和Sargassum sp.）中酚类化合物提取的效果。其中，Sargassum sp.的乙醇提取物含有最高含量的总酚类（25.33±1.45 mg GAE/g），并表现出对2,2-二苯基-1-苦基肼自由基的强效清除活性（33.65±0.03 mg TE/g）和磷钼酸盐还原能力（52.98±0.47 mg TE/g）。Ecklonia sp.的甲醇提取物中含有最高含量的总黄酮类（0.40±0.02 mg QE/g），而其乙酸乙酯提取物中含有最高含量的总缩合单宁（8.09±0.12 mg CE/g）。Fucus sp.表现出相对较强的抗氧化活性，其中甲醇提取物对2,2'-联氨基苯并噻唑磺酸自由基的清除能力为54.41±0.24 mg TE/g，水提取物对还原铁离子的抗氧化能力为36.24±0.06 mg TE/g。同样，液相色谱-质谱联用技术鉴定了61种单个的酚类化合物，包括17种酚酸类化合物、32种黄酮类化合物和12种其他多酚类化合物。Ecklonia sp.的乙醇提取物尤其具有最多样化的组成。这些发现强调了根据具体海藻品种和所需化合物选择适当溶剂的重要性，并进一步为制药、营养保健和化妆品行业提供了有价值的指导。实际应用：酚类化合物是陆地植物和海洋生物体内存在的次生代谢物，由于其潜在的健康优势和多样的生物学效应，受到了广泛关注。在提取过程中使用不同的有机/无机溶剂，可以选择性地从海藻中分离出不同类型的酚类化合物。每种溶剂的独特极性和溶解性能使得可以提取特定的化合物，从而促进了对海藻样品中酚类成分的全面评估，并指导了工业生产。 © 2023 Institute of Food Technologists.

Seaweeds, serving as valuable natural sources of phenolic compounds (PCs), offer various health benefits like antioxidant, anti-inflammatory properties, and potential anticancer effects. The efficient extraction of PCs from seaweed is essential to harness their further applications. This study compares the effectiveness of different solvents (ethanol, methanol, water, acetone, and ethyl acetate) for extracting PCs from four seaweed species: Ascophyllum sp., Fucus sp., Ecklonia sp., and Sargassum sp. Among them, the ethanol extract of Sargassum sp. had the highest content of total phenolics (25.33 ± 1.45 mg GAE/g) and demonstrated potent scavenging activity against the 2,2-diphenyl-1-picrylhydrazyl radical (33.65 ± 0.03 mg TE/g) and phosphomolybdate reduction (52.98 ± 0.47 mg TE/g). Ecklonia sp. had the highest content of total flavonoids (0.40 ± 0.02 mg QE/g) in its methanol extract, whereas its ethyl acetate extract contained the highest content of total condensed tannins (8.09 ± 0.12 mg CE/g). Fucus sp. demonstrated relatively strong antioxidant activity, with methanolic extracts exhibiting a scavenging ability against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (54.41 ± 0.24 mg TE/g) and water extracts showing ferric-reducing antioxidant power of 36.24 ± 0.06 mg TE/g. Likewise, liquid chromatography-mass spectrometry identified 61 individual PCs, including 17 phenolic acids, 32 flavonoids, and 12 other polyphenols. Ecklonia sp., particularly in the ethanol extract, exhibited the most diverse composition. These findings underscore the importance of selecting appropriate solvents based on the specific seaweed species and desired compounds, further providing valuable guidance in the pharmaceutical, nutraceutical, and cosmetic industries. PRACTICAL APPLICATION: The PCs, which are secondary metabolites present in terrestrial plants and marine organisms, have garnered considerable attention due to their potential health advantages and diverse biological effects. Using various organic/inorganic solvents during the extraction process makes it possible to selectively isolate different types of PCs from seaweed species. The distinct polarity and solubility properties of each solvent enable the extraction of specific compounds, facilitating a comprehensive assessment of the phenolic composition found in the seaweed samples and guiding industrial production.© 2023 Institute of Food Technologists.