纯化禽白血病病毒J亚群颗粒的蛋白质组学分析。
Proteomic profiling of purified avian leukosis virus subgroup J particles.
发表日期：2023 Jun 22
作者：
Sheng Chen, Yiming Yan, Liguo Gao, Shuang Gao, Keyu Feng, Hongxin Li, Xinheng Zhang, Weiguo Chen, Feng Chen, Qingmei Xie
来源：
VETERINARY MICROBIOLOGY
摘要：
虽然在不同的病毒中已经证明寄生细胞内蛋白在病毒生命周期中的存在和作用，但是在禽白血病病毒（ALV）中，这些特征仍然大部分未知。为了研究在ALV中是否存在这种情况，我们从禽白血病病毒J亚群（ALV-J）中纯化了高完整性和高纯度的病毒，并使用纳米液相色谱串联质谱（nano LC-MS/MS）进行了蛋白质组分析。该分析鉴定出53种细胞蛋白被包装进成熟的ALV-J病毒中，并通过亚精蛋白酶消化和免疫印迹分析验证了包装细胞蛋白的可靠性。功能注释揭示了这些蛋白在病毒生命周期和肿瘤发生中的潜在功能。总的来说，我们的发现对于理解ALV-J与宿主之间的相互作用具有重要意义，并为定义ALV-J感染所需的细胞要求提供了新的见解。版权所有 © 2023 Elsevier B.V. 保留所有权利。
While the presence of host cell proteins in virions and their role in viral life cycles have been demonstrated in various viruses, such characteristics have remained largely unknown in avian leukosis virus (ALV). To investigate whether this is the case in ALV, we purified high-integrity and high-purity virions from the avian leukosis virus subgroup J (ALV-J) and subjected them to proteome analysis using nano LC-MS/MS. This analysis identified 53 cellular proteins that are incorporated into mature ALV-J virions, and we verified the reliability of the packaged cellular proteins through subtilisin digestion and immunoblot analysis. Functional annotation revealed the potential functions of these proteins in the viral life cycle and tumorigenesis. Overall, our findings have important implications for understanding the interaction between ALV-J and its host, and provide new insights into the cellular requirements that define ALV-J infection.Copyright © 2023 Elsevier B.V. All rights reserved.