中国多年来一直传统使用文冠果（Brucea javanica (L.) Merr.）籽粒治疗各种类型的癌症。本研究系统地研究了由文冠果籽粒制备的文冠果油乳剂（BJOE），旨在评估其对乙型肝炎病毒（HBV）的抗病毒作用。使用HepG2.215（乙型肝炎病毒细胞系）、HepG2和转染了野生型（WT）或拉米夫定耐药突变型（LMV-MT）HBV复制子质粒的Huh7细胞进行不同剂量的BJOE处理，并用于药效学评价。使用CCK8试验确定细胞存活率。评估细胞培养上清液中的HBsAg/HBeAg、细胞裂解液中的HBcAg以及两者中的HBV DNA水平。BJOE在≤5 mg/ml浓度下对癌细胞系无毒性，但可以通过上调白细胞介素-6（IL-6）显著抑制WT/LMV-MT HBV的复制和HBs/e/c抗原的表达，呈剂量依赖性，表明具有适度的抗-HBV活性。作为BJOE的主要成分之一，文冠果碱B在IL-6诱导和HBV抑制中发挥了主导作用。我们的结果表明，BJOE通过刺激IL-6抑制了HBV的复制，表明它在WT和LMV-MT HBV的临床治疗潜力方面具有前景。© 2023 Qin, Shen, Lai, Yang, Feng and Ding版权所有。

The seeds of Brucea javanica (L.) Merr. (BJ) have been traditionally used to treat various types of cancers for many years in China. In this study, we systematically investigated a BJ oil emulsion (BJOE) produced from BJ seeds with the purpose of evaluating its antiviral effect against hepatitis B virus (HBV).HepG2.215 (a wild-type HBV cell line), HepG2, and Huh7, transfected with wildtype (WT) or lamivudine-resistance mutant (LMV-MT) HBV replicon plasmids, were treated with different doses of BJOE and then used for pharmacodynamic evaluation. Cell viability was determined using CCK8 assay. The levels of HBsAg/HBeAg in cell cultured supernatant, HBcAg in cell lysis solution, and HBV DNA in both were evaluated.BJOE at ≤5 mg/ml was nontoxic to carcinoma cell lines, but could significantly inhibit WT/LMV-MT HBV replication and HBs/e/c antigen expression in a dose-dependent manner by upregulating interleukin-6 (IL-6), demonstrating that it possesses moderate anti-HBV activity. As one of the major components of BJOE, bruceine B was found to play a dominant role in IL-6 induction and HBV inhibition.Our results demonstrated that BJOE suppressed HBV replication by stimulating IL-6, indicating that it has promising clinical therapeutic potential for both WT and LMV-MT HBV.Copyright © 2023 Qin, Shen, Lai, Yang, Feng and Ding.