人类结直肠息肉中LncPVT1和CircPVT1的识别作为预后生物标志物。
Identification of LncPVT1 and CircPVT1 as prognostic biomarkers in human colorectal polyps.
发表日期：2023 Aug 12
作者：
Mahsa RezaSoltani, Flora Forouzesh, Zahra Salehi, Mohammad-Reza Zabihi, Leili Rejali, Ehsan Nazemalhosseini-Mojarad
来源：
MOLECULAR & CELLULAR PROTEOMICS
摘要：
LncPVT1和CircPVT1是PVT1基因的异构体，并与癌症进展和癌变有关。本研究调查了结肠腺瘤息肉中LncPVT1和CircPVT1的表达。我们取了40个结直肠息肉组织和40个正常邻近组织(NATs)。通过qRael-Time PCR评估了LncPVT1和CircPVT1的表达。研究了表达与临床病理特征的关系。使用LncPVT1和CircPVT1、预测的miRNAs和miRNAs靶点构建了ceRNA网络。接下来，使用cytoHubba软件包确定了这个网络中的中心节点。在息肉和NATs中区分了过表达的LncPVT1和CircPVT1。LncPVT1和CircPVT1的表达水平在腺瘤息肉中显著高于增生性息肉。LncPVT1和CircPVT1的ROC曲线下面积分别为0.74和0.77。观察到LncPVT1表达与CircPVT1呈正相关。在ceRNA网络中确定了三种miRNAs (hsa-mir-484, hsa-mir-24-3p, hsa-mir-423-5p)与CircPVT1有关。本研究表明，LncPVT1和CircPVT1的表达谱在癌前息肉中显著升高。此外，基于我们的in silico分析，LncPVT1、CircPVT1/miR-484、miR-24-3p、miR-423-5p/PLAGL2轴可能参与结肠癌的发展。在患有癌前结直肠息肉的患者中，LncPVT1和CircPVT1可以作为潜在的预后生物标志物。© 2023 Springer Nature Limited.
LncPVT1 and CircPVT1 are isoforms for the PVT1 gene and are associated with cancer progression and carcinogenesis. Our study investigated the expression of LncPVT1 and CircPVT1 in colon adenoma polyps. 40 tissues of colorectal polyps and 40 normal-adjacent tissues (NATs) were taken. The expression of LncPVT1 and CircPVT1 was evaluated through qRael-Time PCR. The relation between expression and features of clinicopathological was explored. The ceRNA network was constructed by LncPVT1 and CircPVT1 and predicted miRNAs and miRNAs targets. Further, hub nodes in this network were determined using the cytoHubba package. Over-expressed LncPVT1 and CircPVT1 were differentiated in polyp and NATs. The expression level of LncPVT1 and CircPVT1 were significantly higher in adenoma polyps than in hyperplastic polyps. The area under the curve of the ROC estimate for the LncPVT1 and CircPVT1 was 0.74 and 0.77, respectively. A positive correlation was observed between the LncPVT1 expression and CircPVT1. Three miRNAs, including hsa-miR-484, hsa-miR-24-3p, hsa-miR-423-5p, and CircPVT1, were detected as ceRNA hub nodes. In this study, expression profiles of LncPVT1 and CircPVT1 were significantly higher in precancerous polyps. In addition, based on our in silico analysis, LncPVT1, CircPVT1/miR-484, miR-24-3p, miR-423-5p/PLAGL2 axis might be involved in colon cancer development. LncPVT1 and CircPVT1 can be prescribed as warning problems as potential prognostic biomarkers in patients with pre-CRC colon polyps.© 2023. Springer Nature Limited.