通过RNA测序或DNA甲基化微阵列分析，得出3组和4组髓母细胞瘤样本的连续性评分。
Deriving a continuum score for group 3 and 4 medulloblastoma tumor samples analyzed via RNA-sequencing or DNA methylation microarray.
发表日期：2023 Aug 11
作者：
James P R Hacking, Dean Thompson, Edward C Schwalbe, Steven C Clifford, Daniel Williamson
来源：
MOLECULAR & CELLULAR PROTEOMICS
摘要：
在这里，我们提供了一种通过RNA测序或DNA甲基化微阵列分析的3组和4组小脑母细胞瘤肿瘤样本派生连续评分的方案。我们描述了利用非负矩阵分解定义的3组/4组元基因来计算0到1之间的连续评分的步骤，该评分可以通过RNA测序分析的新样本数据进行投影。然后，我们详细介绍了使用随机森林分类器来逆向工程通过DNA甲基化微阵列分析的样本的连续评分的过程。
版权所有 © 2023 作者。由Elsevier Inc.出版。保留所有权利。
Here, we present a protocol for deriving a continuum score for group 3 and 4 medulloblastoma tumor samples analyzed via RNA-sequencing or DNA methylation microarray. We describe steps for utilizing NMF-defined group 3/group 4 metagenes to calculate a continuum score between 0 and 1 that can be projected onto new sample data analyzed via RNA-sequencing. We then detail procedures for reverse engineering a continuum score for samples analyzed via DNA methylation microarray using a random forest classifier.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.