乳腺癌是女性死因中最常见的原因之一。荧光标记是癌细胞检测中一种必不可少的研究和诊断工具。开发廉价且易获取的荧光探针用于癌细胞检测具有重要意义。本文报道了一种绿色且廉价的从紫甘蓝中提取天然花色素荧光剂的方法，并展示其在利用无共价结合天然花色素荧光剂和曲妥珠单抗于人表皮生长因子受体2（HER2）阳性乳腺癌细胞的荧光生物成像中的应用。在该工作中，所提取的花色素经傅里叶变换红外光谱（FTIR）、紫外可见光谱（UV-Vis）和荧光光谱表征。所提取的花色素提取物显示出适合于显微荧光细胞成像的荧光强度，几乎没有光漂白现象，并且没有细胞毒性迹象（细胞存活率超过90%）。花色素结构中的羟基和羧基官能团为花色素与抗体的非共价结合提供了机会。通过将天然花色素荧光剂与曲妥珠单抗非共价结合制备的荧光探针用于乳腺癌细胞上HER2受体的特异性荧光成像。这种绿色荧光探针可能在生物诊断和生物成像技术中有多种未来应用。版权 © 2023. Elsevier Inc. 发表。

Breast cancer is one of the most common causes of death among women. Fluorescent labeling is an essential research and diagnostic tool in the detection of cancer cells. The development of inexpensive and easily accessible fluorescent probes for the detection of cancerous cells is of great importance. Herein we report a green and inexpensive method for extraction of natural anthocyanin fluorophore from Red Cabbage and demonstrate its application for fluorescent bioimaging of human epidermal growth factor receptor 2 (HER2) positive breast cancer cells using non-covalent conjugation of anthocyanin fluorophores to Trastuzumab antibody. In this work, the extracted anthocyanins were characterized by Fourier transform infrared spectroscopy (FTIR), Ultraviolet-Visible (UV-Vis) and fluorescent spectroscopy. The anthocyanin extract showed proper fluorescent intensity for microscopic fluorescent cell imaging, negligible photobleaching and no sign of cytotoxicity (more than 90% viability). The presence of hydroxy and carboxyl functional groups in the structure of anthocyanins provided the opportunity for the non-covalent conjugation of anthocyanins to antibodies. The fluorescent probe made by non-covalent conjugation of the anthocyanin fluorophores to Trastuzumab antibody was used for specific fluorescent imaging of HER2 receptors on HER2 positive breast cancer cells. This green fluorescent probe may have several future applications in biological diagnosis and bio-imaging techniques.Copyright © 2023. Published by Elsevier Inc.