卵母细胞老化直接影响其后续的胚胎发育。茺蔻毛叶籽提取物中的活性成分是伞形菊酯乙醇，具有潜在的抗癌、抗炎和抗氧化特性。此外，伞形菊酯乙醇能够减缓人皮肤成纤维细胞的老化。为了探讨伞形菊酯乙醇对离体培养成熟的猪卵母细胞老化的影响，首先建立了老化模型，将伞形菊酯乙醇添加到离体成熟培养基中，通过观察卵母细胞的成熟和胚胎发育潜力，以及分析与老化相关的基因表达、活性氧和线粒体膜电位水平来研究其抗老化效应。研究发现，在离体培养的66小时后，猪卵母细胞出现典型的老化现象。与44小时组相比，66小时组和72小时组的卵黄周围空间更大，微管形成异常增加，囊胚率显著降低。与0μg/mL组相比，10μg/mL组的首体胞排出率、分裂率和囊胚率显著提高（P < 0.05）。卵母细胞发育潜力相关的、SIRT家族相关的、抗氧化和抗凋亡相关基因的表达显著上调（P < 0.05），p53和促凋亡基因显著下调（P < 0.05）。此外，10μg/mL组的活性氧水平显著降低（P < 0.01），线粒体膜电位显著增高（P < 0.01）。总之，伞形菊酯乙醇通过上调SIRT家族基因表达，增强卵母细胞的抗氧化和抗凋亡能力，延缓了体外培养的猪卵母细胞的老化。版权所有 © 2023. Elsevier Ltd出版。

Oocyte aging directly affects the subsequent embryonic development. Epifriedelanol is the active ingredient of Aster tataricus L.F. extract, and it possesses potential anti-cancer, anti-inflammatory and antioxidant properties. In addition, epifriedelanol can slow the aging of human skin fibroblasts. To explore the effect of epifriedelanol on the aging of porcine oocytes matured in vitro, the aging model was first established, epifriedelanol was added to in vitro maturation (IVM) medium to investigate its anti-aging effects by observing oocyte maturation and embryonic development potential, and analyzing aging-related gene expression, reactive oxygen species and mitochondrial membrane potential levels. It was found that typical aging of porcine oocytes appeared from 66 h during in vitro maturation. Compared with the 44 h group, a larger perivitelline space, increased abnormality of microtubulin formation, and significantly lower blastocyst rate were observed in the 66 h and 72 h groups. Compared with the 0 μg/mL group, the first polar body extrusion, cleavage and blastocyst rates were significantly improved (P < 0.05) in 10 μg/mL group. The expression of oocyte developmental potential-related, SIRT family-related, antioxidant and anti-apoptotic-related genes was significantly up-regulated (P < 0.05), p53 and pro-apoptotic genes were significantly down-regulated (P < 0.05). In addition, the reactive oxygen species level was significantly decreased (P < 0.01), the mitochondrial membrane potential was significantly elevated (P < 0.01) in 10 μg/mL group. In conclusion, epifriedelanol delays the aging of porcine oocytes cultured in vitro by up-regulating SIRT family gene expression, enhancing the antioxidant and anti-apoptotic capacity of oocytes.Copyright © 2023. Published by Elsevier Ltd.