为了将二甲双胍装载到纳米配方中并评估该产生的纳米形式对癌细胞的作用，二甲双胍被装载到天然载体椰子油上。所形成的二甲双胍-椰子油纳米乳液通过Zeta电位、颗粒大小、药物含量、药物释放和药物稳定性进行表征。所形成的纳米乳液对MCF-7、HepG2和HCT-116细胞系进行了评估。细胞周期分析和凋亡机制进行了研究。在超声波细胞破碎器中使用去离子水、1.5% Spna 20、1.5% Tween 80、1.5% 椰子油和0.5% 二甲双胍制造了均匀溶液来创建纳米乳液。与非配方二甲双胍相比，装载二甲双胍的椰子油纳米乳液的抗癌活性显著提高，MCF-7、HepG2和HCT-116细胞系的IC50分别为8.3±0.1 µg/ml、12±1.5 µg/ml、2.685±0.3 µg/ml。经纳米乳液处理后，MCF-7和HepG2细胞的凋亡细胞数量分别增加了76.5±2.3%和78.3±3.2%。该配方可被视为一种新型的抗癌药物。

In order to load metformin in a nano formula and evaluate the produced nano form towards cancer cells, metformin was loaded on natural carrier coconut oil. The formed metformin-loaded coconut oil nanoemulsion was characterized by Zeta potential, particle size, drug content, drug release, and drug stability. The formed nanoemulsion was evaluated towards MCF-7, HepG2, and HCT-116 cell lines. Cell cycle analysis and apoptosis mechanism were studied. The nanoemulsion was created using deionized water, 1.5% Span 20, 1.5% Tween 80, 1.5% coconut oil, and 0.5% Metformin in an ultrasonicator to produce a homogenous solution. The anticancer activities of the metformin-loaded coconut nanoemulsion were highly improved compared to non-formulated metformin with IC50s of 8.3 ± 0.1 µg/ml, 12 ± 1.5 µg/ml, 2.685 ± 0.3 µg/ml for MCF-7, HepG2, and HCT-116 cell lines, respectively. There was a 76.5 ± 2.3 and 78.3 ± 3.2% increase in the number of apoptotic cells of MCF-7 and HepG2 cells after nanoemulsion treatment. This formula may be considered a new anticancer medication.