乳腺癌是世界上女性中最常见的癌症类型之一。miR-301b-3p/5p是来自相同前体miRNA的成对miRNA，可能在肿瘤中具有不同的临床作用，并需要进一步的探索和研究。为了研究miR-301b-3p/5p的差异表达、临床意义、诊断和预后价值，并探索它们在乳腺癌中的功能，我们从TCGA数据集中提取了有关miRNA的信息进行临床相关性分析，并通过GO、KEGG富集和免疫渗透分析来探索其潜在功能。miR-301b-3p/5p在乳腺癌中都高度表达，它们之间存在正相关。miR-301b-3p和miR-301b-5p具有不同的临床特征。在乳腺癌中，miR-301b-3p可以作为潜在的诊断标志物，而miR-301b-5p可以作为预后分子。GO、KEGG富集和免疫渗透分析表明，miR-301b-3p主要聚焦于分子功能，miR-301b-5p主要聚焦于血管生成调控，并且与免疫细胞相关。miR-301b-3p和miR-301b-5p在乳腺癌中均具有肿瘤促进作用，miR-301b-3p可以作为潜在的诊断标志物，而miR-301b-5p可以作为预后分子和潜在的治疗靶点。尽管miR-301b-3p/5p是同一前体miRNA的两个分支miRNA，它们可能通过不同的途径共同促进乳腺癌的进展。© 2023 The Authors.

Breast cancer is one of the most common cancer type of women in the world. miR-301b-3p/5p were paired miRNAs derived from the same pre-miRNA, which may have different clinical roles in tumor and requires more exploration and research.In order to investigate the differential expression, clinical significance, diagnostic and prognostic value of miR-301b-3p/5p and explore their function in breast cancer, we extracted information of miRNAs from TCGA data sets for clinical correlation analysis, and the potential function was explored by GO、KEGG enrichment and immunoinfiltration analysis.miR-301b-3p/5p were both highly expressed in breast cancer, there is a positive correlation between them. miR-301b-3p and miR-301b-5p have different clinical features. In breast cancer, miR-301b-3p can be used as a potential diagnostic marker while miR-301b-5p can be used as a prognostic molecule. GO, KEGG enrichment and immunoinfiltration analysis reveals that miR-301b-3p focuses on molecular functions, miR-301b-5p focuses on regulation of angiogenesis, and it is correlated with immune cells.miR-301b-3p and miR-301b-5p are both tumor promoter in breast cancer, miR-301b-3p can be used as a potential diagnostic marker, while miR-301b-5p can be used as a prognostic molecule and an underlying therapy target. Although miR-301b-3p/5p is a pair of miRNAs from two arms of the same pre-miRNA, they may promote the progression of breast cancer together through different pathway.© 2023 The Authors.