pH变化是许多病理学的标志，包括癌症和肾病。在这里，我们介绍了作为超极化细胞外pH和灌注传感器的[1,5-13C2]Z-OMPD，用于MRI，它允许生成肾脏疾病状态的多参数指纹，并检测局部肿瘤的酸化。在1T下，T1长达两分钟，pH敏感性高达每个pH单位1.9ppm，使用C1标签作为内部频率参考的适用性使得在健康大鼠肾脏中实现体内pH成像成为可能。对13C-共振的光谱选择性靶向使得在三维中同时成像灌注和过滤，同时在二维中成像pH，以在一分钟内定量健康和积水性肾脏的肾血流量、肾小球滤过率和肾脏pH，比临床常规方法具有更高的灵敏度。单个会话中成像多种生物标志物使[1,5-13C2]Z-OMPD成为肿瘤学和肾脏学的一种有前景的新型超极化剂。© 2023. Springer Nature Limited.

pH alterations are a hallmark of many pathologies including cancer and kidney disease. Here, we introduce [1,5-13C2]Z-OMPD as a hyperpolarized extracellular pH and perfusion sensor for MRI which allows to generate a multiparametric fingerprint of renal disease status and to detect local tumor acidification. Exceptional long T1 of two minutes at 1 T, high pH sensitivity of up to 1.9 ppm per pH unit and suitability of using the C1-label as internal frequency reference enables pH imaging in vivo of three pH compartments in healthy rat kidneys. Spectrally selective targeting of both 13C-resonances enables simultaneous imaging of perfusion and filtration in 3D and pH in 2D within one minute to quantify renal blood flow, glomerular filtration rates and renal pH in healthy and hydronephrotic kidneys with superior sensitivity compared to clinical routine methods. Imaging multiple biomarkers within a single session renders [1,5-13C2]Z-OMPD a promising new hyperpolarized agent for oncology and nephrology.© 2023. Springer Nature Limited.