本文描述了一种用于检测人类血浆样本中突变的microRNA的定量检测方法。根据Peyrard-Bishop模型设计的特异性寡核苷酸能够准确预测目标：探针识别亲和力和特异性。我们的放大自由串联珠基因组杂交测定法的检测限为2.2 pM。因此，该测定法能够在临床相关的microRNA-128-2-3p中识别单核苷酸多态性不匹配的基因型，显示与炎症性结肠炎和结直肠癌呈正相关的末端突变。版权：© 2023 Slott 等。本文是一篇遵循知识共享署名许可的开放获取文章，允许在任何媒介中无限制使用、分发和复制，前提是保持原始作者和出处的署名。

We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer.Copyright: © 2023 Slott et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.