结直肠癌（CRC）是一种异质性疾病，临床结果各异。基于分子分析鉴定CRC患者的不同亚组可以有助于更好地了解疾病和改善患者结果。本研究旨在调查膜运输相关基因（MTRGs）在基于总生存和免疫微环境的分类结直肠癌患者中的潜力。共识聚类分析鉴定出两个不同的聚类，具有不同的膜运输相关基因表达谱。群集1中的患者的总生存显著优于群集2。此外，两个聚类中的免疫微环境也被发现存在显著差异，群集1具有更高的免疫得分和更多的免疫细胞存在。对两个聚类之间的差异表达基因进行功能分析表明MTRGs参与了免疫反应和代谢过程，并建立了基于MTRGs的风险签名模型来预测CRC患者的预后。这些发现表明，MTRGs在CRC患者的免疫微环境和总生存中起着关键作用，可能为个体化治疗提供潜在靶点。© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Colorectal cancer (CRC) is a heterogeneous disease with varying clinical outcomes. The identification of distinct subgroups of CRC patients based on molecular profiling can aid in better understanding the disease and improving patient outcomes. This study aimed to investigate the potential of membrane trafficking-related genes (MTRGs) in sub-grouping colorectal cancer patients based on their overall survival and immune microenvironments. Consensus clustering analysis identified two distinct clusters with different expression profiles of membrane trafficking-related genes. The patients in cluster 1 had a significantly better overall survival than those in cluster 2. Furthermore, the immune microenvironments in the two clusters were also found to be significantly different, with cluster 1 having a higher immune score and more immune cells present. Functional analysis of differentially expressed genes between the two clusters revealed that MTRGs were involved in immune response and metabolic processes, and a risk signature model based on MTRGs was established to predict the prognosis of CRC patients. These findings suggest that MTRGs play a crucial role in the immune microenvironment and overall survival of CRC patients and may provide a potential target for personalized therapy.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.