尽管存在实际的复杂性，人体同位素示踪研究变得越来越可行。然而，在充分利用人体示踪研究之前，需要解决一些技术上的挑战。首先，由于组织切除仅限于单一手术时间点，因此，鼠标中的绝对代谢通量测量并不容易应用于人体模型。其次，同位素示踪尚未被用于在体内检测细胞类型之间的代谢差异。在这里，我们讨论了当前的模型，并提出了一种替代液态肿瘤环境，以克服这些限制。此外，我们还强调了目前用于在细胞分离技术后维持同位素标记富集度的策略，以促进细胞类型特异性分析。版权所有 © 2023 Elsevier Ltd. 保留所有权利。

Despite practical complexities, isotope tracing studies in humans are becoming increasingly feasible. However, several technological challenges need to be addressed in order to take full advantage of human tracing studies. First, absolute metabolic flux measurements in mice are not so easily applied to human models, given that tissue resection is restricted to a single surgical time point. Second, isotope tracing has yet to be employed to detect metabolic differences between cells types in vivo. Here, we discuss the current models and propose an alternative, liquid tumor environment, that could overcome these limitations. Furthermore, we highlight current strategies used to maintain isotopolog enrichment following cell isolation techniques to facilitate cell-type-specific analysis.Copyright © 2023 Elsevier Ltd. All rights reserved.