从不同地点采集的菟丝子（Hypericum ternatum Poulter）和染痕山金钱（H. scabrum L.）物种的精油成分和生物活性鉴定：染痕山金钱是多功能物种圣约翰草（H. perforatum）的替代选择吗？
Determination of essential oil and biological activities of Hypericum ternatum Poulter and H. scabrum L. species collected from different localities: is H. scabrum an alternative to multifunctional species ST JOHN'S WORT (H. perforatum)?
发表日期：2022
作者：
Mehmet Akdeniz, İsmail Yener, Sevgi Irtegün Kandemir, Şafak Özhan Kocakaya, Mehmet Firat, Serkan Yiğitkan, Nesrin Haşimi, Abdulselam Ertaş, Ufuk Kolak
来源：
ANTIOXIDANTS & REDOX SIGNALING
摘要：
随着时间的推移，作为传统药材而被使用的金丝桃Hypericum L.物种在科学界的重要性日益增加。金丝桃物种是一类长期以来被用于治疗多种疾病并具有疗效的植物。本研究通过GC-MS/FID法，确定了土耳其不同地区采集的剑叶金丝桃Hypericum scabrum L.和三叶金丝桃H. ternatum Poulter的精油成分。此外，还研究了它们的抗氧化、细胞毒性和抗微生物活性以及酶抑制潜能（胆碱酯酶、脲酶、酪氨酸酶、弹性蛋白酶和胶原酶）。同时，在研究的酶上进行了物种主要成分的体外和体内研究。结果发现，四个剑叶金丝桃样品主要含有α-蒎烯（55.99%-62.80%），而三个三叶金丝桃样品则含有2-甲基辛烷（9.45%-22.39%）和α-蒎烯（12.75%-33.08%）。剑叶金丝桃精油除抗微生物活性外具有明显的生物活性潜力，而三叶金丝桃样品没有。所有的剑叶金丝桃精油样品在结肠癌细胞株（HT-29）上表现出较高的细胞毒性效果（IC50 21.67 ± 0.34 至 34.67 ± 0.45 μg/mL），并且显示出比标准化合物加兰他敏更高的乙酰胆碱酯（抑制率 83.30 ± 1.90 至 93．08±1.04）和丁酰胆碱酯（抑制率 80.58 ± 1.19 至 93.28 ± 1.99）抑制活性。此外，所有的剑叶金丝桃样品均显示出较高的酪氨酸酶（抑制率 69.00 ± 1.64 至 95.25 ± 1.42）和弹性蛋白酶（抑制率 27.58 ± 0.34 至 36.69 ± 0.18）抑制效果。这些生物活性结果表明，剑叶金丝桃精油可以在化妆品和制药行业中使用。© TÜBİTAK.
The importance of Hypericum L. species, being used in traditional medicine, in the scientific world is increasing day by day. Hypericum species are plants that have been used in the treatment of many diseases for a long time and have healing properties. In the current study, the essential oil compositions of Hypericum scabrum L. and H. ternatum Poulter collected from different localities in Turkey were determined by GC-MS/FID. In addition, their antioxidant, cytotoxic, and antimicrobial activities with their enzyme inhibitory potentials (cholinesterase, urease, tyrosinase, elastase, and collagenase) were investigated. Also, in vitro and in silico studies of the major components of the species have been carried out on the studied enzymes. It was determined that four H. scabrum samples mainly contained α-pinene (55.99%-62.80%) while three H. ternatum samples contained 2-methyloctane (9.45%-22.39%) and α-pinene (12.75%-33.08%). While H. scabrum essential oils possessed significant biological activity potential except for the antimicrobial activity, H. ternatum samples did not. All essential oil samples of H. scabrum exhibited a high cytotoxic effect (IC50 21.67 ± 0.34 to 34.67 ± 0.45 μg/mL) against colon cancer cell line (HT-29) and indicated higher acetyl-(Inhibiton% 83.30 ± 1.90 to 93.08 ± 1.04) and butyryl-cholinesterase (Inhibiton% 80.58 ± 1.19 to 93.28 ± 1.99) inhibitory activity than the standard compound, galantamine. Furthermore, all samples of H. scabrum showed high tyrosinase (Inhibiton% 69.00 ± 1.64 to 95.25 ± 1.42) and elastase (Inhibiton% 27.58 ± 0.34 to 36.69 ± 0.18) inhibitory effects. These biological activity results indicated that H. scabrum essential oil could be used in the cosmetic and pharmaceutical industries.© TÜBİTAK.