为了研究 NOP16 在前列腺癌（PCa）中的作用，该基因是雌激素和 c-Myc 的靶基因。采用定量实时聚合酶链反应（qRT-PCR）和免疫印迹分别检测了 PCa 和良性前列腺增生（BPH）组织以及 PC-3 和 RWPE-1 细胞株中的 NOP16 表达。使用免疫组化法（IHC）评估了 BPH 和 PCa 蜡包埋组织中的 NOP16 表达。利用癌症基因组图谱（TCGA）数据库分析了 NOP16 基因表达对 PCa 患者无病生存的影响。使用 CCK-8、划痕愈合、Transwell 和流式细胞术分别评估了体外敲低 NOP16 后的 PC-3 细胞增殖、迁移、侵袭和凋亡的变化。采用基因本体论（GO）和基因集富集分析（GSEA）分析了在 PC-3 细胞中敲低 NOP16 后的 RNA 测序数据。采用小鼠模型探究了 NOP16 对 PC-3 皮下肿瘤生长的影响。NOP16 在 PCa 组织中的表达显著高于 BPH 组织，且在 PC-3 细胞中的表达显著高于 RWPE-1 细胞。NOP16 表达低的 PCa 患者的无病生存时间较长。核糖体生物合成、细胞外基质受体相互作用、PTEN 和 VEGF 信号通路可能在 NOP16 敲低组中发生了改变。敲低 NOP16 能显著抑制 PC-3 细胞在体外和体内的增殖、迁移和侵袭。NOP16 低表达可减少 PC-3 细胞的总蛋白质合成并诱导凋亡。NOP16 可望成为预测 PCa 发生和发展的新生物标志物，并成为 PCa 的治疗靶点。© 2023 临床科学家协会

To investigate the role of NOP16, a target gene of estrogen and c-Myc, in prostate cancer (PCa).The expressions of NOP16 in PCa and benign prostate hyperplasia (BPH) tissues, PC-3 and RWPE-1 cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot respectively. The expressions of NOP16 in BPH and PCa paraffin-embedded tissues were assessed by immunohistochemistry (IHC). The Cancer Genome Atlas (TCGA) database was used to analyze the effect of NOP16 gene expression on the disease-free survival of PCa patients. CCK-8, wound healing, transwell assays and flow cytometric analysis were used to assess the changes of proliferation, migration, invasion and apoptosis of PC-3 cells respectively after knocking down NOP16 in vitro. Gene ontology (GO) and gene set enrichment analysis (GSEA) were used to analyze the RNA sequencing data followed by knocking down NOP16 in PC-3 cells. Mouse models were used to explore the effect of NOP16 on PC-3 subcutaneous tumor growth in vivo.The NOP16 expression was significantly higher in PCa tissues than that in BPH tissues and significantly higher in PC-3 cells than in RWPE-1 cells. PCa patients with low NOP16 expression have a longer disease-free survival than that with high NOP16 expression. Ribosome biogenesis in eukaryotes, ECM-receptor interaction, PTEN and VEGF signaling pathways may be changed in the NOP16 knockdown group than control. Knockdown of NOP16 could significantly inhibit the proliferation, migration and invasion of PC-3 cells in vitro and in vivo. Low expression of NOP16 could reduce the total protein synthesis and induce the apoptosis of PC-3 cells.NOP16 may be expected to be a novel biomarker for predicting the occurrence and development of PCa, and may become a target for the treatment of PCa.© 2023 by the Association of Clinical Scientists, Inc.