真菌感染与高发病率和高死亡率相关，尤其在患有慢性肺病、HIV、癌症和糖尿病等基础健康问题的患者中高度流行。为了减轻这些感染，开发有效的抗真菌药物是至关重要的，而植物作为生物活性化合物的有希望的来源显得突出。在本研究中，我们着重研究蕺菜（Lavandula multifida）精油对皮肤癣菌菌株和白色念珠菌的抗生物膜潜力。利用气相色谱和质谱对精油进行了表征，并对其抗真菌效果进行了生物膜形成和破坏的评估。通过结晶紫、苏芹啉和XTT试验，定量测定了生物膜质量、胞外基质和细胞活力，并利用光学和扫描电子显微镜证实了形态学改变。蕺菜精油含有极高量的芳樟酚，并对真菌生物膜的抑制和破坏非常有效。精油显著降低了所有被测试真菌的生物膜质量和细胞活力。此外，在皮肤癣菌生物膜形成过程中观察到了真菌的胞外基质减少。成熟生物膜中明显观察到了形态学改变，菌丝直径显著减小。这些有希望的结果支持在开发有效的基于植物的抗真菌产品中使用蕺菜精油。

Fungal infections are associated with high morbidity and mortality rates, being highly prevalent in patients with underlying health complications such as chronic lung disease, HIV, cancer, and diabetes mellitus. To mitigate these infections, the development of effective antifungals is imperative, with plants standing out as promising sources of bioactive compounds. In the present study, we focus on the antibiofilm potential of Lavandula multifida essential oil (EO) against dermatophyte strains and Candida albicans. The EO was characterized using GC and GC-MS, and its antifungal effect was assessed on both biofilm formation and disruption. Biofilm mass, extracellular matrix, and viability were quantified using crystal violet, safranin, and XTT assays, respectively, and morphological alterations were confirmed using optical and scanning electron microscopy. L. multifida EO showed very high amounts of carvacrol and was very effective in inhibiting and disrupting fungal biofilms. The EO significantly decreased biofilm mass and viability in all tested fungi. In addition, a reduction in dermatophytes' extracellular matrix was observed, particularly during biofilm formation. Morphological alterations were evident in mature biofilms, with a clear decrease in hypha diameter. These promising results support the use of L. multifida EO in the development of effective plant-based antifungal products.