NUCB-2/Nesfatin-1与鼻咽癌（NPC）的关联仍然不清楚。我们阐明了NUCB-2/Nesfatin-1在NPC的发展、进展和诊断中的作用。在鼻咽癌细胞系（5-8F，6-10B，CNE1，CNE2和NP69）中，进行了Western blotting，MTT，EdU和其他技术的实验，以研究NUCB-2在鼻咽癌中的作用。共收集了70个组织样本（39个NPC和31个鼻炎）和140个血清样本（包括NPC、鼻炎、其他头颈部肿瘤和健康对照），探讨了鼻咽癌患者组织或血清中NUCB-2及其代谢产物Nesfatin-1的表达情况。NPC组织中NUCB-2水平高于鼻炎组织（P < 0.05）。抑制NPC细胞系CNE2中的NUCB-2可以抑制细胞的增殖和克隆形成；相反，增加NPC细胞系CNE1中的NUCB-2会促进细胞的繁殖和克隆发育。与患有其他头颈部肿瘤，鼻炎或健康供体的患者相比，鼻咽癌患者的血清NUCB-2水平升高。在NPC诊断中，血清样本中nesfatin-1与EA-IgA，VCA-IgA和Rta-IgG的测定能够达到敏感性93.6%和特异性94.5%，而这种诊断模型的阳性和阴性预测值分别为89.8%和96.6%，准确性为94.2%。本研究揭示了NUCB-2可以增强NPC细胞的增殖，NUCB-2/Nesfatin-1有潜力成为辅助早期诊断鼻咽癌的血清标志物。 © 2023. BioMed Central Ltd., part of Springer Nature.

The association of NUCB-2/Nesfatin-1 with nasopharyngeal carcinoma (NPC) remains unclear. We clarified the role of NUCB-2/Nesfatin-1 in the development, progression and diagnosis of NPC.In nasopharyngeal carcinoma cell lines (5-8 F, 6-10B, CNE1, CNE2 and NP69), western blotting, MTT, EdU and other techniques were performed to investigate the role of NUCB-2 in nasopharyngeal carcinoma. 70 tissue samples (39 NPC and 31 rhinitis) and 140 serum samples (including NPC, rhinitis, other head and neck tumors and healthy control) were included to explore the expression of NUCB-2 and its metabolite Nesfatin-1 in tissues or serum of patients with nasopharyngeal carcinoma.NUCB-2 level in NPC tissue was higher than that in rhinitis tissue (P < 0.05). Suppression of NUCB-2 in the NPC cell line CNE2 inhibited proliferation and clone formation of the cells; on the contrary, improvement of NUCB-2 in the NPC cell line CNE1 promoted cell propagation and clone development. An elevated serum level of NUCB-2 in NPC patients was detected, compared to that in patients with other head and neck tumors, rhinitis or healthy donors. Determination of nesfatin-1 combined with EA-IgA, VCA-IgA and Rta-IgG in serum samples for NPC diagnosis reached a sensitivity of 93.6% and a specificity of 94.5%, while the positive and negative predictive value of this diagnostic model was 89.8% and 96.6%, and the accuracy yielded 94.2%.This study revealed that NUCB-2 could enhance proliferation of NPC cells and NUCB-2/nesfatin-1 has the potential to be a serological marker to aid early diagnosis of nasopharyngeal carcinoma.© 2023. BioMed Central Ltd., part of Springer Nature.