组织的病理学诊断依赖于组织学成像的黄金标准，但由于繁琐的样本制备过程，可能妨碍对新生物的诊断和管理所需的关键信息的及时获取。无需染色的未经处理标本中，幻灯片无需捕捉细胞核是更理想的选择；然而，新鲜组织中不可避免的表面不规则会导致一定局限性。本文提出了一种紫外亚表面，具有生成保持<1.1微米横向分辨率和约290微米焦深的紫外光学聚焦能力，可用于快速、高分辨率、无标记的光声组织学成像，可用于未经处理的组织样本，包括表面不平整的组织。通过手工切割的小鼠脑样本的实验证明，可以观察到细胞核的微观解剖特征，对于大约3×3毫米的视野，成像时间大约为27分钟。因此，预计紫外亚表面辅助的光声显微镜将有助于改善繁琐的组织制备工作，从而有益于术中病理评估和基础科学研究。© 2023 作者。

Pathology interpretations of tissue rely on the gold standard of histology imaging, potentially hampering timely access to critical information for diagnosis and management of neoplasms because of tedious sample preparations. Slide-free capture of cell nuclei in unprocessed specimens without staining is preferable; however, inevitable irregular surfaces in fresh tissues results in limitations. An ultraviolet metasurface with the ability to generate an ultraviolet optical focus maintaining < 1.1-µm in lateral resolution and ∼290 µm in depth of field (DOF) is proposed for fast, high resolution, label-free photoacoustic histological imaging of unprocessed tissues with uneven surfaces. Microanatomical characteristics of the cell nuclei can be observed, as demonstrated by the mouse brain samples that were cut by hand and a ∼3 × 3-mm2 field of view was imaged in ∼27 min. Therefore, ultraviolet metasurface-assisted photoacoustic microscopy is anticipated to benefit intraoperative pathological assessments and basic scientific research by alleviating laborious tissue preparations.© 2023 The Authors.