本研究旨在研究基质金属蛋白酶-8（MMP-8）基因型在结直肠癌（CRC）发展中的参与作用。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术分析362例CRC患者和362例对照组的MMP-8 C-799T（rs11225395）、Val436Ala（rs34009635）和Lys460Thr（rs35866072）基因型。此外，还评估了这些基因型与年龄、性别、吸烟、饮酒和体重指数（BMI）等因素在CRC风险方面的潜在关联。对MMP-8 rs11225395基因型分布在对照组和病例组之间未发现显著差异（趋势性p = 0.3836）。 Logistic回归分析表明MMP-8 rs11225395变异CT和TT基因型患者的CRC风险分别为0.83和0.77倍。此外，携带rs11225395 CT+TT基因型与CRC风险无关（p = 0.2063）。此外，在非吸烟者中，MMP-8 rs11225395 TT基因型个体相比CC基因型个体显著降低了CRC风险（p = 0.0379）。MMP-8 rs34009635或rs35866072方面未观察到显著关联。分析表明，MMP-8的基因型在决定个体对CRC风险的敏感性方面起到次要作用。版权所有 ©2023国际抗癌研究所（George J. Delinasios博士），保留所有权利。

This study aimed to investigate the involvement of matrix metalloproteinase-8 (MMP-8) genotypes in the development of colorectal cancer (CRC).The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to analyze the genotypes of MMP-8 C-799T (rs11225395), Val436Ala (rs34009635), and Lys460Thr (rs35866072) in 362 patients with CRC and 362 controls. Additionally, the potential associations between these genotypes and factors such as age, sex, smoking, alcohol consumption, and body mass index (BMI) status in relation to CRC risk were also assessed.No significant differences in the distribution of MMP-8 rs11225395 genotypes were found between the control and case groups (p for trend=0.3836). Logistic regression analysis demonstrated that individuals with the MMP-8 rs11225395 variant CT and TT genotypes had a 0.83 and 0.77-fold risk of CRC, respectively. Moreover, carriers of the rs11225395 CT+TT genotypes were not associated with CRC risk either (p=0.2063). Furthermore, individuals with the MMP-8 rs11225395 TT genotype exhibited significantly lower odds of CRC risk compared to those with the CC genotype among non-smokers (p=0.0379). No significant associations were observed with respect to MMP-8 rs34009635 or rs35866072.The analyzed genotypes of MMP-8 play a minor role in determining individual susceptibility to CRC risk.Copyright © 2023 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.