靶向和摄取是增强癌症光热疗法（PTT）疗效和减少对周围正常组织损伤的最重要策略。本研究制备了一种基于纳米气泡和氮化钛的纳米光敏剂，用于肝细胞癌的协同治疗。通过膜水合法将光热剂氮化钛（TiN）包裹在纳米气泡中，并在细胞和动物中进行验证。使用CCK-8、细胞死亡染色和JC-1验证光热联合超声靶向纳米气泡破裂（UTND）的有害效应，然后通过尾静脉注射到动物体内观察其光热效应和体内抑制效果。溶血试验和体重变化验证其安全性。新型纳米光敏剂的平均直径为300.3 ± 12.7 nm，具有一致的纳米球形态。利用UTND技术通过超声辐照的物理能量提高了TiN进入肿瘤细胞的穿透力。通过体外和体内实验证明了UTND和PTT的协同治疗效果。本研究确定NBs@C3F8-TiN作为适用于超声光热治疗肝细胞癌的合适超声光热剂，其具有适当的尺寸和高效的光热效果。版权所有 © 2023 Elsevier Inc. 保留所有权利。

Targeting and uptake are the most important strategies for enhancing the efficacy of cancer photothermal therapy (PTT) and reducing damage to surrounding normal tissues. In this study, a kind of nanophotosensitizer based on nanobubbles and TiN was prepared for synergetic therapy for hepatocellular carcinoma.The photothermal agent titanium nitride (TiN) was wrapped in nanobubbles by membrane hydration method and verified in cells and animals. CCK-8, cell death staining, and JC-1 were used to verify the pernicious effect of photothermal combined with Ultrasound Targeted Nanobubble Destruction (UTND) and then injected into animals through the tail vein to observe its photothermal effect and in vivo inhibitory effect. A hemolysis test and body weight change verified its safety.The average diameter of the novel nanophotosensitizer was 300.3 ± 12.7 nm, with a consistent nanospheres morphology. The UTND technology was utilized to improve the penetration of TiN into tumor cells through the physical energy of ultrasound irradiation. The therapeutic effects of the synergistic therapy of UTND and PTT were verified in vitro and in vivo.The research has established NBs@C3F8-TiN as a suitable ultrasound photothermal agent due to its appropriate size and efficient photothermal efficacy for visual photothermal therapy for HCC.Copyright © 2023 Elsevier Inc. All rights reserved.