本研究旨在开发和测试一种生物传感器，用于检测伊拉克捐赠者血样中的放射性氡气和铅离子。该生物传感器由适配体，乙酸，孔雀绿和三茂基氯胺组成，使用荧光分光光度计测定结果。研究发现血液中的222Rn在个体之间存在差异，男性和吸烟者的水平较高，患有癌症的男性患者含量最高。用于检测血液中222Rn的生物传感器具有有效，敏感和低成本的特点，并且检测到的水平在世界卫生组织设定的限值之内。研究还研究了有毒金属pb+2，并发现其含量在允许范围内。生物传感器也可以有效检测pb+2。变量之间的相关性一般较弱到中等，湿度与其他变量之间存在负相关关系。温度（Tin）和温度（Tout）之间存在一些强正相关关系。结果表明这些变量之间没有强相关性，这对于了解它们对健康结果的潜在影响是一项重要发现。然而，在临床设置中广泛使用之前，需要进一步的验证和测试。本研究强调了监测血液中这些物质的重要性，特别是对于职业暴露于辐射的人员。与其他检测设备相比，研究发现该生物传感器敏感，具有成本效益，制造快速和高效。因此，建议在血样中测量氡和铅离子时使用该生物传感器。本研究使用的生物传感器可能是这种监测的有用工具。© 2023 Elsevier Ltd.发表。

This study aimed to develop and test a biosensor for detecting radioactive radon gas and lead ions in blood samples collected from donors in Iraq. The biosensor was made up of aptamer, acetic acid, malachite green, and TRIS-HAC, and results were measured using a fluorescence spectrophotometer. This study found that 222Rn in the blood varied between individuals, with higher levels in males and smokers, and the highest concentration found in a male patient with cancer. The biosensor used to detect 222Rn in the blood was effective, sensitive, and low-cost, and the levels detected were within the limits set by the WHO. The study also looked at pb+2, a toxic metal, and found that levels were within permissible limits. The biosensor was also effective in detecting pb+2. The correlations between the variables are generally weak to moderate, and there are some negative relationships between humidity and other variables. There are also some strong positive relationships between temperature (Tin) and temperature (Tout). The results suggest that these variables are not strongly correlated with each other, which is an important finding for understanding their potential effects on health outcomes. However, further validation and testing may be necessary before its widespread use in clinical settings. This study highlights the importance of monitoring these substances in the blood, especially for individuals with occupational exposure to radiation. The biosensor was found to be sensitive, cost-effective, fast to manufacture, and efficient compared to other detection devices. Therefore, the study recommends the use of this biosensor for measuring radon and lead ions in blood samples. The biosensor used in this study could be a useful tool for such monitoring.© 2023 Published by Elsevier Ltd.