可持续纳米颗粒源于北沙参，并对前列腺癌二维和三维模型的抗癌潜力进行分析。
Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer.
发表日期：2023 Sep 08
作者：
Prachi Vaid, Adesh K Saini, Raju Kumar Gupta, Eshu Singhal Sinha, Deepak Sharma, Walaa F Alsanie, Vijay Kumar Thakur, Reena V Saini
来源：
Cellular & Molecular Immunology
摘要：
为了寻找一种新型有效的前列腺癌治疗方法，利用白花乌头茎块提取物（Sg-ME）制备了银（Sg-AgNP）、氧化铜（Sg-CuONP）和银-铜双金属纳米颗粒（Sg-BNP）。纳米颗粒的表征结果证实Sg-AgNP、Sg-CuONP和Sg-BNP的平均直径分别为30.72、32.19和25.59纳米，呈现球形。有趣的是，这些纳米颗粒对前列腺癌（PC3）细胞系表现出显著的细胞毒性，而对正常细胞不具有毒性。纳米颗粒能够通过增强活性氧（ROS）产生和线粒体去极化来诱导PC3细胞凋亡。此外，在治疗这些绿色纳米颗粒后的4天内观察到3D前列腺肿瘤球体的收缩。与2D模型系统相比，3D模型系统对纳米颗粒的敏感性较低。Sg-BNP在2D和3D前列腺癌模型上展示出最高的抗癌潜力。© 2023. 作者，独家许可给Springer Science+Business Media, LLC，并属于Springer Nature。
In pursuit of a novel effective treatment for prostate cancer, methanolic extract of Stephania glabra tubers (Sg-ME) was utilized to fabricate silver (Sg-AgNP), copper oxide (Sg-CuONP), and silver-copper bimetallic nanoparticles (Sg-BNP). The characterization of the nanoparticles confirmed spherical shape with average diameters of 30.72, 32.19, and 25.59 nm of Sg-AgNP, Sg-CuONP, and Sg-BNP, respectively. Interestingly, these nanoparticles exhibited significant cytotoxicity toward the prostate cancer (PC3) cell line while being non-toxic toward normal cells. The nanoparticles were capable of inducing apoptosis in PC3 cells by enhancing reactive oxygen species (ROS) generation and mitochondrial depolarization. Furthermore, the shrinkage of 3D prostate tumor spheroids was observed after 4 days of treatment with these green nanoparticles. The 3D model system was less susceptible to nanoparticles as compared to the 2D model system. Sg-BNP showed the highest anticancer potential on 2D and 3D prostate cancer models.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.