具有高催化活性的单原子纳米酶（SAzymes）展示了使肿瘤微环境（TME）中的反应性氧代谢平衡失调的潜力。该环境含有多种内源性还原性物质，如谷胱甘肽（GSH）。本文首次构建了一种新型纳米装配体（CDs@Pt SAs/NCs@DOX），使用负载铂（Pt）单原子或纳团簇纳米酶与药物预处理，其中Pt负载量为34.8%。该装配体展示出杰出的双酶活性，模拟过氧化酶（POD）和谷胱甘肽氧化酶（GSHOx）。独特的GSHOx样活性能以较低的Km（1.04 mm）和较高的Vmax（7.46 × 10-6 m s-1 ）有效清除GSH，避免单一氧（1 O2）的消耗。CDs@Pt SAs/NCs@DOX同时表现出低温光热疗法和TME-或激光控制的解聚和药物释放能力，可有效调节细胞氧化还原平衡，实现肿瘤生长的高度抑制。这些结果为制备具有多种活性和可变尺寸的Pt SAzymes纳米装配体提供了有希望的策略，从而拓宽了SAzymes和纳米装配体在生物医学领域的应用前景。© 2023 The Authors. Advanced Science由Wiley-VCH GmbH出版。

Single-atom nanozymes (SAzymes) with high catalytic activity exhibit the potential to disequilibrate the reactive oxygen metabolic balance in the tumor microenvironment (TME), which contains several endogenous reductive substances such as glutathione (GSH). Herein, a novel nano-assembly (CDs@Pt SAs/NCs@DOX) is first constructed using drug-primed platinum (Pt) single-atom or nanocluster nanozymes with a Pt loading of 34.8%, which exhibits prominent dual enzymatic activities to mimic peroxidase (POD) and glutathione oxidase (GSHOx). The unique GSHOx-like activity can efficiently scavenge GSH with a relatively low Km (1.04 mm) and high Vmax (7.46 × 10-6  m s-1 ), thus avoiding single oxygen (1 O2 ) depletion. CDs@Pt SAs/NCs@DOX simultaneously demonstrates low-temperature photothermal therapy and TME- or laser-controlled disassembly and drug release, which can effectively regulate cellular redox homeostasis and achieve high tumor growth inhibition. These outcomes may provide promising strategies for the preparation of Pt SAzymes with multiple activities and variable-sized nano-assemblies, allowing for broader applications of SAzymes and nano-assemblies in the biomedical field.© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.