天然药物领域迫切需要定向分离活性植物化合物。本文利用纤维素的天然多孔性和高生物相容性，成功制备了一种纳米纤维素膜结合表面分子印迹技术；高效能的纳米纤维素分子印迹膜（NC-MIM）能够很好地吸附目标分离的植物化合物，如癌症药物紫杉醇合成中的重要中间体10-去乙酰紫杉醇Ⅲ（10-DAB）。通过一系列表征和吸附实验，确定了NC-MIM的吸附机制。在pH 8.0和20 °C-40 °C的温度下，NC-MIM对10-DAB的最大吸附容量达到66.90 mg g - 1，吸附后10-DAB的含量显著增加17.5倍。具体的吸附结果显示，NC-MIM对于从紫杉醇结构类似物中定向分离10-DAB具有优秀的能力。即使经过十个循环，NC-MIM仍表现出卓越的吸附能力，达到86.43 %，表明其选择性和稳定性异常出色。NC-MIM通过对植物中活性植物化合物的绿色、安全和高效富集的成功实现，为这一领域提供了一种有前景的新方法，并提供了宝贵的实际应用见解。版权所有 © 2023. Elsevier B.V.出版。

Targeted separation of active phytochemicals is urgently needed in the natural medicine field. In this paper, due to the natural porosity and high biocompatibility of cellulose, a nanocellulose membrane combined with surface molecular imprinting was successfully prepared; the efficient nanocellulose-based molecular imprinted membrane (NC-MIM) provided good adsorption for the targeted separation of phytochemicals such as 10-deacetylbaccatin III (10-DAB), an essential intermediate in the synthesis of the anticancer drug paclitaxel. Through a series of characterization and adsorption experiments, the adsorption mechanism of NC-MIM was determined. At pH 8.0 and temperatures of 20 °C-40 °C, the maximum capacity of NC-MIM for adsorption of 10-DAB reached 66.90 mg g - 1, and the content of 10-DAB was dramatically increased 17.5-fold after adsorption. The specific adsorption results showed that NC-MIM had excellent capacity for targeted separation of 10-DAB from among taxane structural analogues. Even after ten cycles, NC-MIM demonstrated a remarkable adsorption capacity of 86.43 %, thereby indicating exceptional selectivity and stability. The successful implementation of NC-MIM for green, safe, and efficient enrichment of phytochemicals from plants provides a promising new approach and valuable insights into its practical application.Copyright © 2023. Published by Elsevier B.V.