细胞的特性描述和操控在生物学和医学应用中起着重要作用。细胞存活度评估对于细胞毒理学试验、抗癌药剂剂量测试和其他生化刺激具有重要意义。细胞的电性质在正常变为病理状态时会发生变化。目前评估细胞存活度的方法通常需要一个复杂的芯片，而且通量有限。本文通过使用交流电动力学开发了一种基于双极电极阵列的微流控装置，用于评估细胞存活度。通过分析细胞的电转动（ROT）速度和方向以及细胞的电介质电泳（DEP）响应，可以评估多种细胞（包括酵母细胞和K562细胞）的存活度。首先，可以通过细胞在双极电极上被捕获的位置来识别细胞存活度，通过DEP力。此外，还可以通过ROT来评估细胞的存活度，根据细胞的旋转速度和方向。在行波介电电泳力的作用下，还可以通过细胞在双极电极边缘上的旋转运动来区分细胞的存活度。该研究证明了使用双极电极的AC电动力学平台在芯片设计方面具有出色的灵活性，从而使其能够实现可扩展和高通量的应用。通过使用双极电极，我们提出的新技术在细胞特性和存活度评估的原位检测和分析中具有广泛应用。版权所有© 2023 Elsevier B.V. 保留所有权利。

Cell characterization and manipulation play an important role in biological and medical applications. Cell viability evaluation is of significant importance for cell toxicology assay, dose test of anticancer drugs, and other biochemical stimulations. The electrical properties of cells change when cells transform from healthy to a pathological state. Current methods for evaluating cell viability usually requires a complicated chip and the throughput is limited.In this paper, a bipolar electrode (BPE) array based microfluidic device for assessing cell viability is exploited using AC electrodynamics. The viability of various cells including yeast cells and K562 cells, can be evaluated by analyzing the electro-rotation (ROT) speed and direction of cells, as well as the dielectrophoresis (DEP) responses of cells. Firstly, the cell viability can be identified by the position of the cell captured on the BPE electrode in terms of DEP force. Besides, cell viability can also be evaluated based on both the cell rotation speed and direction using ROT. Under the action of travelling wave dielectric electrophoresis force, the cell viability can also be distinguished by the rotational motion of cells on bipolar electrode edges.This study demonstrates the utility of BPEs to enable scalable and high-throughput AC electrodynamics platforms by imparting a flexibility in chip design that is unparalleled by using traditional electrodes. By using BPEs, our proposed new technique owns wide application for cell characterization and viability assessment in situ detection and analysis.Copyright © 2023 Elsevier B.V. All rights reserved.