依托于对Lentinus edodes中天然药用产品Lentinan的极小副作用，Lentinan制剂被认为是癌症治疗的一种天然药物。然而，Lentinus edodes很容易遭受不可降解重金属，特别是银离子（Ag+）的污染。因此，高度希望能够监测Lentinus edodes中Ag+的污染情况，充分考虑其对Lentinan制剂的不良影响。电化学传感器不受基质浑浊和色彩干扰的影响，为测定不同分析物提供了强有力的手段。针对Ag+的电化学传感，需要设计高效的信号探针来进行特异识别和信号产生。本文提出了一种基于血红素-包埋金属有机框架（PorMOF）的类生物催化和寡C碱基DNA的变构开关的适当电化学适体传感器用于Ag+的测定。由于良好的生物相容性，PorMOF被用作通过加载双链DNA支架来设计信号探针的纳米酶类物质。由于Ag+与富含胞嘧啶（C）碱基的DNA的特异性识别，远端的PorMOF通过C-Ag+-C结构与下面的电极相邻，增强了催化羟胺氧化的电流，从而产生了更高的信号。根据电流响应和Ag+浓度之间的正相关关系，该电化学系统提供了一种在线监测Lentinus edodes中Ag+的有前景的手段，其回收率为92.8%至106.4%，相对标准偏差为3.98%至8.24%。验证了该电化学适体传感器在Lentinus edodes中对Ag+的适用性。由于便携性、操作简单和快速响应等优点，该电化学模式为Lentinus edodes中Ag+的在线监测提供了一个有用的解决方案。通过改变DNA序列，所提出的适体传感器为监测其他重金属提供了一种强有力的方式，能够保护药物制剂免受重金属污染的影响。版权所有 © 2023 Elsevier B.V.。保留所有权利。

Lentinan medicament from Lentinus edodes has been considered as natural medicinal products with minimal side effects for cancer therapy, but Lentinus edodes are easily polluted by nonbiodegradable heavy metals, especially silver ion (Ag+). Therefore, it is highly desirable to monitor Ag + pollution in Lentinus edodes considering their adverse impact on lentinan medicament. Electrochemical sensor isn't affected from the interference of matrix turbidity and color, and offers a powerful means for determination of variant analytes. As for electrochemical sensing toward Ag+, there is a great need to design efficient signal probes for specific recognition and signal generation.We present an appropriate electrochemical aptasensor for Ag + assay based on biomimetic catalysis of porphyrin-encapsulated MOF (PorMOF) and allosteric switch of C-rich DNA. Thanks to the excellent biocompatibility, PorMOFs as nanozyme are used to design signal probes by loading duplex-like DNA scaffold. Owing to the specific recognition of Ag+ toward cytosine (C) base-rich DNA, PorMOF at the distal end was close to the underlying electrode via C-Ag+-C formation, leading to an enhanced current of catalytic hydroxylamine oxidation for signal generation. Using the positive correlation between current response and Ag+ level, the electrochemical system provides a promising means for on-line monitoring of Ag+ in Lentinus edodes with recoveries from 92.8% to 106.4% and relative standard deviation from 3.98% to 8.24%, verifying the applicability of the electrochemical aptasensor toward Ag+ in Lentinus edodes.With merits of portability, simple operation, and rapid response, the electrochemical pattern offers a useful solution for on-line monitoring of Ag+ in Lentinus edodes. By altering the DNA sequence, the proposed aptasensor provides a powerful way for monitoring other heavy metals, capable of protecting medicament production from heavy metal pollution.Copyright © 2023 Elsevier B.V. All rights reserved.