为了研究葡萄糖氧化酶载荷和基于锰的介孔二氧化硅纳米颗粒（MSN@Mn-GOx）的物化特征，以及其对胃肠癌的抗肿瘤效应。我们使用透射电子显微镜（TEM）、动态光散射（DLS）、Zeta电位分析、紫外吸收光谱、能量色散光谱和X射线光电子能谱等方法对MSN@Mn-GOx纳米颗粒的形态、粒径和类似Fenton反应的特性进行了分析。我们建立了人结肠癌HT-29异体移植小鼠模型，利用MRI技术检查了MSN@Mn-GOx的抗肿瘤效应。我们使用反应性氧自由基（ROS）产生实验、CCK-8实验和EdU实验评估了纳米颗粒的体外抗肿瘤效应。MSN@Mn-GOx纳米颗粒呈固体球状，直径约为100 nm，Zeta电位为-35 mV。在含有葡萄糖的溶液中，MSN@Mn-GOx对H2O2的催化效率高于不含葡萄糖的溶液，在 pH6.0时的Fenton-like特性强于 pH7.4时（P<0.05）。在肿瘤携带的小鼠中，MSN@Mn-GOx治疗剂量依赖地增强了肿瘤的T1成像（P<0.01）。与对照组和MSN@Mn组相比，MSN@Mn-GOx诱导了明显更高水平的ROS产生，并对胃癌和结肠癌细胞的增殖有较强的抑制作用（P<0.05）。MSN@Mn-GOx纳米颗粒具有良好的化学动力学特性和强大的抗肿瘤效应，并为胃癌提供了潜在的治疗选择。

To investigate the physicochemical features of glucose oxidase-loaded and manganese-based mesoporous silica nanoparticles (MSN@Mn-GOx) and its antitumor effect against gastrointestinal cancer.The morphology, particle size and Fenton-like properties of MSN@Mn-GOx nanoparticles were analyzed using transmission electron microscopy (TEM), dynamic light scattering (DLS), Zeta potential analysis, ultraviolet absorption spectroscopy, energy dispersive spectroscopy and X-ray photoelectron spectroscopy. A mouse model bearing human colon cancer HT-29 xenograft was established to examine the antitumor effect of MSN@Mn-GOx using MRI imaging. Reactive oxygen species (ROS) production assay, CCK-8 assay and EdU assay were used to evaluate the in vitro anti-tumor effect of the nanoparticles.MSN@Mn-GOx nanoparticles were solid spheres with a diameter of about 100 nm and a Zeta potential of -35 mV. MSN@Mn-GOx had a higher H2O2 catalytic efficiency in glucose containing solution than in glucose-free solution, and showed a stronger Fenton-like properties at pH6.0 than at pH7.4 (P＜0.05). In the tumor-bearing mice, MSN@Mn-GOx treatment dose-dependently enhanced T1 imaging of the tumor (P＜0.01). Compared with the control group and MSN@Mn group, MSN@Mn-GOx induced a significantly higher level of ROS production and a stronger inhibitory effect on the proliferation of gastric and colon cancer cells (P＜0.05).MSN@Mn-GOx nanoparticles have good chemodynamic properties and a strong anti-tumor effect and provide a potential therapeutic option for gastric cancer.