在本研究中，我们试图利用甜橙（Citrus sinensis）果皮提取物（CS-Ni/ZnO NCs）制备镍/氧化锌纳米复合材料，并研究其在胃癌 AGS 细胞中阻断 JAK/STAT3 信号通路的能力。我们采用紫外-可见光谱分析、傅里叶变换红外光谱、X射线衍射、场发射扫描电子显微镜、能量色散X射线谱分析、动态光散射和电泳电位等不同方法对制备的 CS-Ni/ZnO NCs 进行表征。通过使用适当的荧光探针描述程序测量 ROS、MMP 和细胞凋亡的情况，检验了 CS-Ni/ZnO NCs 的抗癌潜力对 AGS 细胞。合成的 CS-Ni/ZnO NCs 显示出一个直径为74.76 nm 的棒状结构。细胞毒性实验显示，CS-Ni/ZnO NCs 显著减少了 AGS 细胞的存活率，且呈剂量依赖性。荧光探针实验结果显示，CS-Ni/ZnO NCs 引起了 AGS 细胞的凋亡。JAK/STAT-3 的过表达被认为扩展了增殖和抗凋亡的转录调节。因此，阻断 JAK/STAT-3 的表达被认为是抑制胃癌增殖扩张的关键目标。CS-Ni/ZnO NCs 治疗成功阻断了 AGS 细胞中的 JAK/STAT3 信号级联通路，同时也启动了细胞凋亡通路。研究结果表明，CS-Ni/ZnO NCs 可能成为治疗胃癌的有前景的化学预防剂。利用甜橙果皮提取物制备了镍/氧化锌纳米复合材料（CS-Ni/ZnO NCs），并通过紫外-可见光谱、X射线衍射、傅里叶变换红外光谱和透射电子显微镜对其进行了表征以确认纳米粒子的特征。CS-Ni/ZnO NCs 通过诱导 ROS 介导的 MMP 改变和细胞凋亡特征，在 AGS 胃癌细胞中产生了这些效应。CS-Ni/ZnO NCs 抑制了 JAK/STAT3 信号通路，从而抑制了胃癌细胞的增殖并诱导了细胞凋亡。© 2023. 作者，独家授权给 Springer Science+Business Media, LLC 中的一部分 Springer Nature。

In this study, we sought to fabricate nickel/zinc oxide nanocomposites utilizing Citrus sinensis (C. sinensis) peel extract (CS-Ni/ZnO NCs) and investigate their ability to impede the JAK/STAT3 signaling pathway in gastric cancer AGS cells. Different methods, including UV-Vis spectral analysis, FT-IR, XRD, FE-SEM, EDAX, DLS, and zeta potential, were used to characterize the fabricated CS-Ni/ZnO NCs. By measuring ROS, MMP, and apoptotic cell death using the appropriate fluorescence describing procedures, the anticancer potential of CS-Ni/ZnO NCs was examined against AGS cells. The synthesized CS-Ni/ZnO NCs displayed a rod structure with a diameter of 74.76 nm. The cytotoxicity assay showed that the CS-Ni/ZnO NCs diminished the viability of the AGS cells in a dosage-dependent manner. Results from the fluorescence probe assay showed that the CS-Ni/ZnO NCs caused apoptosis in AGS cells. JAK/STAT-3 over expressions thought to expand the transcriptional regulation of proliferation and anti-apoptosis. Hence, inhibition of JAK/STAT-3 expression is considered a crucial target for impeding the expansion of gastric cancer proliferation. The JAK/STAT3 signaling cascade was successfully blocked by CS-Ni/ZnO NCs treatment, which also started the apoptotic pathway in the AGS cells. The findings conclude that CS-Ni/ZnO NCs might serve as a promising chemo-preventive agent for treating GC. Nickel/Zinc oxide nanocomposites were synthesized using C. sinensis peel extract (CS-Ni/ZnO NCs) and characterized by UV-Vis, XRD, FTIR, and TEM to confirm the nanoparticles. CS-Ni/ZnO NCs induce the overproduction of ROS-mediated MMP alteration and apoptosis features in AGS gastric cancer cells. CS-Ni/ZnO NCs inhibited the JAK/STAT3 signaling, thereby suppressing the proliferation and inducing apoptosis in gastric cancer cells.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.