耐药性三阴性乳腺癌（TNBC）是这种疾病的一种亚型，对传统化疗药物具有耐药性。 IFN-τ是一种细胞因子，最近被证明具有免疫调节和抗肿瘤作用。本研究旨在探讨IFN-τ对乳腺癌细胞的抗增殖和凋亡作用以及在TNBC小鼠肿瘤模型中的抗肿瘤作用。培养并用羊IFN-τ处理小鼠乳腺癌4T1细胞，并通过MTT和Caspase进行处理-Glo 3/7 测定、活力和细胞死亡被确定。此外，IFN-τ的抗肿瘤作用在TNBC小鼠肿瘤模型中得到确定。绵羊IFN-τ对4T1小鼠乳腺癌细胞显示出浓度依赖性的抗增殖作用。此外，用 IFN-τ 处理 4T1 细胞会诱导 caspase 3 和 7 的激活，这表明细胞凋亡。此外，我们检测到用 IFN-处理的细胞中 I 型干扰素受体 (IFNAR1/2) 的表达增加。与对照未治疗组相比，小鼠瘤内应用 IFN-τ 可抑制肿瘤生长，且该作用与 GM-CSF 表达增加有关。绵羊 IFN-τ 可能是治疗 TNBC 的有效免疫治疗细胞因子.版权所有 © 2023，国际抗癌研究所（George J. Delinasios 博士），保留所有权利。

Resistant triple-negative breast cancer (TNBC) is a subtype of this disease that is resistant to conventional chemotherapy agents. IFN-τ is a cytokine that has recently been shown to have immunoregulatory and antitumor effects. The present study aimed to examine the antiproliferative and apoptosis effects of IFN-τ in breast cancer cells and the antitumor effect in a murine tumor model of TNBC.Murine breast cancer 4T1 cells were cultured and treated with ovine IFN-τ and through MTT and Caspase-Glo 3/7 assays, viability and cell death were determined. In addition, the antitumor effect of IFN-τ was determined in a murine tumor model of TNBC.Ovine IFN-τ showed a concentration-dependent antiproliferative effect on 4T1 murine breast cancer cells. Also, treatment of 4T1 cells with IFN-τ induced the activation of caspase 3 and 7, which is indicative of apoptotic cell death. Moreover, we detected an increase in the expression of type I interferon receptor (IFNAR1/2) in cells treated with IFN-. The intratumoral application of IFN-τ in mice inhibited tumor growth compared to the control non-treated group, and the effect was associated with the increased expression of GM-CSF.Ovine IFN-τ may be an effective immunotherapeutic cytokine for the treatment of TNBC.Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.