成簇规则间隔短回文重复序列 (CRISPR) 和 CRISPR 相关蛋白 9 (Cas9) (CRISPR/Cas9) 系统是一种强大的工具，可实现精确、高效的基因操作。在相对较短的时间内，CRISPR 因其高效、简单、可编程性和低成本而成为首选的基因编辑系统。此外，近年来，CRISPR工具包一直在迅速扩展，新兴的进展在揭示人类疾病的分子机制和新的治疗策略方面显示出巨大的潜力。在这篇综述中，我们就 CRISPR 技术的最新进展及其对精准医学的影响（从目标识别、疾病建模和诊断）提供了我们的观点。我们还讨论了表观基因组、碱基编辑和 Prime 编辑等新方法对临床前癌症药物发现的影响。版权所有 © 2023 Ravichandran 和 Maddalo。

The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen to become the preferred gene-editing system due to its high efficiency, simplicity, and programmability at low costs. Furthermore, in the recent years, the CRISPR toolkit has been rapidly expanding, and the emerging advancements have shown tremendous potential in uncovering molecular mechanisms and new therapeutic strategies for human diseases. In this review, we provide our perspectives on the recent advancements in CRISPR technology and its impact on precision medicine, ranging from target identification, disease modeling, and diagnostics. We also discuss the impact of novel approaches such as epigenome, base, and prime editing on preclinical cancer drug discovery.Copyright © 2023 Ravichandran and Maddalo.