简介 肝细胞癌 (HCC) 是世界上最常见的癌症之一，除非及时诊断，否则治疗选择有限。对氧磷酶 (PON) 是一种糖基化蛋白质，与抗氧化和其他生化功能有关。对氧磷酶 1 (PON1) 是一种与高密度脂蛋白 (HDL) 颗粒相关的酯酶。本研究旨在评估 PON1 活性，并将其与标准肝功能测试 (LFT) 进行比较，以评估诊断为 HCC 的患者肝损伤的可预测性。方法 这项病例对照研究在安得拉邦斯里卡库勒姆大东方医学院和医院附属生物化学科进行。在 30 名诊断为 HCC 的患者和 30 名健康人中评估了血清 PON1 活性和 LFT，如总胆红素、直接胆红素、丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、碱性磷酸酶 (ALP)、总蛋白和白蛋白。所有参数均使用标准生化方法进行估计。使用 GraphPad Prism 6.0 版（GraphPad Software, Inc）分析数据。概率 (p) 值 <0.05 被认为具有统计显着性。进行受试者工作特征曲线 (ROC) 分析，以评估曲线下面积 (AUC) 的准确性、敏感性、特异性和诊断效率。结果 PON1 的血清活性与白蛋白 (70%) 具有相同的敏感性 (70%)，并且优于其他测试参数。此外，PON1 活性的特异性 (86.67%) 低于其他测试参数。 ROC 分析显示，与总胆红素 (DE=76.6%；p=0.0039)、直接胆红素 (DE=74.9%；p=0.04) 相比，PON1 的诊断功效 (DE=78.3%；p<0.0001) 有所提高。 ALT（DE=73.30%；p=0.0006）和总蛋白（DE=71.6%；p=0.0005）。然而，PON1 的 DE 与 AST（DE=81.60%；p<0.0001）、ALP（DE=79.9%；p<0.0001）和白蛋白（DE=83.30%，p<0.0001）相当。结论 PON1 血清活性可作为评估 HCC 患者肝损伤的诊断标志物。版权所有 © 2023，Bade 等人。

Introduction Hepatocellular carcinoma (HCC) is one of the most common cancers in the world and unless diagnosed timely has limited options for treatment. Paraoxonase (PON) is a glycosylated protein that has been implicated in antioxidant and other biochemical functions. Paraoxonase 1 (PON1) is an esterase associated with high-density lipoprotein (HDL) particles. The present study was carried out to assess the PON1 activity and compare it with the standard liver function tests (LFTs) in assessing the predictability of liver damage among patients diagnosed with HCC. Methods This case-control study was carried out in the Department of Biochemistry attached to Great Eastern Medical School and Hospital, Srikakulam, Andhra Pradesh. Serum PON1 activities and LFTs like total bilirubin, direct bilirubin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total protein, and albumin were estimated in 30 patients diagnosed with HCC and 30 healthy persons. All the parameters were estimated using standard biochemical methods. The data was analyzed using GraphPad Prism version 6.0 (GraphPad Software, Inc). A probability (p) value <0.05 was considered to be statistically significant. Receiver operating characteristic curve (ROC) analysis was performed to assess the area under the curve (AUC) for accuracy, sensitivity, specificity, and diagnostic efficiency. Results The serum activities of PON1 had identical sensitivity (70%) to albumin (70%) and were superior to other tested parameters. Additionally, PON1 activities showed lower specificity (86.67%) than the other tested parameters. ROC analysis showed increased diagnostic efficacy (DE) of PON1 (DE=78.3%; p<0.0001) when compared with total bilirubin (DE=76.6%; p=0.0039), direct bilirubin (DE=74.9%; p=0.04), ALT (DE=73.30%; p=0.0006), and total protein (DE=71.6%; p=0.0005). However, the DE of PON1 was comparable with AST (DE=81.60%; p<0.0001), ALP (DE=79.9%; p<0.0001), and albumin (DE=83.30%, p<0.0001). Conclusions Serum activities of PON1 could be used as a diagnostic marker for assessing liver damage among HCC patients.Copyright © 2023, Bade et al.