Nyctanthes arbor-tristis (N. arbor-tristis) 是一种具有巨大药用价值的植物，植物的各个部分都保留了广泛的药用特性，如抗菌、抗氧化、抗癌和抗炎活性等。植物甾醇是植物的次生代谢产物以其降低胆固醇、增强免疫力和抑制癌细胞形成的能力而闻名。在这项研究中，使用 HPTLC 对植物叶子的甲醇提取物中的β-谷甾醇进行了定性和定量鉴定，β-谷甾醇可增强抗氧化酶并减少氧化应激。我们的研究结果表明，N. arbor-tristis 具有显着浓度的 β-谷甾醇，Rf 值为 0.64 Rf 值。此外，对接和模拟（计算机）研究表明，β-谷甾醇与人类DNA拓扑异构酶I（h-DNA Topo I）具有良好的结合亲和力，并具有抑制其活性的潜力。它可以被重建为 h-DNA Topo I 抑制剂。由 Ramaswamy H. Sarma 传达。

Nyctanthes arbor-tristis (N. arbor-tristis) is a plant of enormous medicinal importance and each part of the plant retained extensive medicinal properties, such as antimicrobial, antioxidant, anticancer and anti-inflammatory activities, etc. Phytosterols are secondary metabolites of plants that are well-known for their ability to reduce cholesterol, boost immunity and inhibit the formation of cancer cells. In this study, β-sitosterol, which boosts antioxidant enzymes and reduces oxidative stress, was qualitatively and quantitatively identified in the methanolic extract of the plant's leaves using the HPTLC. Our findings show that N. arbor-tristis has a significant concentration of β-sitosterol with the 0.64 Rf value. Further, docking and simulation (in-silico) studies have shown that β-sitosterol has good binding affinity with human DNA Topoisomerase I (h-DNA Topo I) and has the potential to inhibit its activity. It can be reconstructed as h-DNA Topo I determent.Communicated by Ramaswamy H. Sarma.