脯氨酰 3-羟化酶 1 (P3H1) 与癌症发展有关，但尚未对 P3H1 进行泛癌分析。本研究首次对P3H1相关的mRNA表达、突变、启动子甲基化、预后意义等方面进行了研究，并通过检索相关文献，探讨了P3H1与临床病理参数、药物敏感性、免疫细胞浸润的关系。数据库包括使用生物信息学工具的癌症基因组图谱 (TCGA)、基因型组织表达 (GTEx)、cBioPortal 和肿瘤免疫评估资源 (TIMER2.0)。研究结果表明，与正常组织相比，大多数肿瘤中 P3H1 的表达存在显着差异，与临床预后密切相关。泛癌 Cox 回归分析显示，P3H1 高表达与脑低级别胶质瘤、肾透明细胞癌、肾上腺皮质癌、肝细胞癌、间皮瘤、肉瘤、葡萄膜黑色素瘤、膀胱尿路上皮癌患者的低总生存率显着相关。肾乳头状细胞癌、肾嫌色细胞癌、胸腺瘤和甲状腺癌。 P3H1 DNA 甲基化与其表达呈负相关。 P3H1 与浸润细胞、免疫相关基因、肿瘤突变负荷、微卫星不稳定性和错配修复显着相关。最后，发现P3H1表达与对九种药物的敏感性之间存在显着相关性。因此，P3H1表达增强与多种肿瘤的不良预后相关，这可能是由于其在肿瘤免疫调节和肿瘤微环境中的作用。这种泛癌分析深入了解了 P3H1 在不同癌症肿瘤发生中的功能，并为后续的进一步深入研究提供了理论基础。© 2023。作者，获得 Springer Science Business Media, LLC 独家许可，施普林格自然的一部分。

Prolyl 3-hydroxylase 1 (P3H1) has been implicated in cancer development, but no pan-cancer analysis has been conducted on P3H1. In this study, for the first time, aspects associated with P3H1, such as the mRNA expression, any mutation, promoter methylation, and prognostic significance, the relationship between P3H1 and clinicopathological parameters, drug sensitivity, and immune cell infiltration were investigated by searching several databases including The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), cBioPortal, and The Tumor Immune Evaluation Resource (TIMER2.0) using bioinformatics tools. The findings indicate significant differential expression of P3H1 in most tumors when compared to normal tissues, with a strong association with clinical prognosis. A pan-cancer Cox regression analysis revealed that high P3H1 expression is significantly associated with low overall survival in patients with brain lower grade glioma, kidney clear cell carcinoma, adrenocortical cancer, liver hepatocellular carcinoma, mesothelioma, sarcoma, uveal melanoma, bladder urothelial carcinoma, kidney papillary cell carcinoma, kidney chromophobe, thymoma, and thyroid carcinoma. A negative correlation was observed between P3H1 DNA methylation and its expression. P3H1 is significantly associated with infiltrating cells, immune-related genes, tumor mutation burden, microsatellite instability, and mismatch repair. Finally, A significant correlation was found between P3H1 expression and sensitivity to nine drugs. Thus, enhanced P3H1 expression is associated with poor prognosis in a variety of tumors, which may be due to its role in tumor immune regulation and tumor microenvironment. This pan-cancer analysis provides insight into the function of P3H1 in tumorigenesis of different cancers and provides a theoretical basis for further in-depth studies to follow.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.