目前的研究重点是肉豆蔻苷对 MCF-7 人乳腺癌 (BC) 细胞的抗癌活性。 BC 是全世界女性中最常见和最常见的恶性疾病。如今，针对乳腺癌使用了各种传统疗法，但仍然是一个主要挑战，因为这些疗法无法区分正常细胞和恶性细胞，而且还具有严重的副作用。因此，需要开发新疗法来降低 BC 相关的发病率和死亡率。肉豆蔻苷是一种 1-烯丙基-5-甲氧基-3, 4-亚甲二氧基苯，是一种主要的活性芳香化合物，存在于各种香料中，如肉豆蔻、肉豆蔻、胡萝卜、肉桂、香芹和一些精油中。肉豆蔻苷具有广泛的作用，包括抗肿瘤、抗氧化和抗菌活性。然而，肉豆蔻苷对人类 BC 细胞的影响在很大程度上仍未被揭示。通过 (4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物和乳酸脱氢酶测定检测，肉豆蔻苷对 MCF-7 细胞的细胞毒性作用呈剂量依赖性增加。使用吖啶橙/溴化乙锭、Hoechst 染色剂和膜联蛋白 V 发现，与对照相比，肉豆蔻苷可显着诱导细胞凋亡。此外，它还能激活细胞抗迁移、细胞内活性氧生成和 G1/S 期细胞周期停滞阶段。此外，通过定量聚合酶链反应和凋亡蛋白（c-PARP、Caspase 9、细胞色素 C、还使用蛋白质印迹分析了 PDI) 表达。总体而言，我们证明肉豆蔻苷可以调节 MCF-7 BC 细胞中的凋亡信号通路。© 2024 Wiley periodicals LLC。

The current study was focused on the anticancer activity of myristicin against MCF-7 human breast cancer (BC) cells. BC is the most common and leading malignant disease in women worldwide. Now-a-days, various conventional therapies are used against BC and still represent a chief challenge because those treatments fail to differentiate normal cells from malignant cells, and they have severe side effects also. So, there is a need develop new therapies to decrease BC-related morbidity and mortality. Myristicin, a 1‑allyl‑5‑methoxy‑3, 4‑methylenedioxybenzene, is a main active aromatic compound present in various spices, such as nutmeg, mace, carrot, cinnamon, parsely and some essential oils. Myristicin has a wide range of effects, including antitumor, antioxidative and antimicrobial activity. Nevertheless, the effects of myristicin on human BC cells remain largely unrevealed. The cytotoxicity effect of myristicin on MCF‑7 cells was increased dose dependently detected by (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Lactate Dehydrogenase assays. Myristicin was found to be significantly inducing the cell apoptosis, as compared to control, using acridine orange/ethidium bromide, Hoechst stain and annexin V. Moreover, it activates cell antimigration, intracellular reactive oxygen species generation and cell cycle arrest in the G1/S phase. In addition, myristicin induces the expression of apoptosis and cell cycle genes (Caspases8, Bax, Bid, Bcl2, PARP, p53, and Cdk1) was demonstrated by quantitative polymerase chain reaction and apoptosis proteins (c-PARP, Caspase 9, Cytochrome C, PDI) expression was also analyzed with western blot. Overall, we illustrated that myristicin could regulate apoptosis signaling pathways in MCF-7 BC cells.© 2024 Wiley Periodicals LLC.