在这项研究中，我们评估了使用四磺酸铝酞菁 (AlPcS4) 的光动力疗法 (PDT) 对 MCF-7 乳腺癌细胞的活力和细胞应激反应的影响。具体来说，我们研究细胞活力、细胞因子产生和应激相关基因表达的变化。实验组包括对照细胞、仅用 AlPcS4 处理的细胞、仅用发光二极管 (LED) 处理的细胞以及联合 PDT 处理的细胞。为了评估这些对细胞活力、细胞因子产生和应激相关基因表达的影响，可以使用 3-[4,5-二甲基噻唑-2-基]-2,5 二苯基四唑溴化物 (MTT) 测定、酶-采用了连锁免疫吸附测定（ELISA）和实时定量PCR（RT-qPCR）。我们的研究结果揭示了 AlPcS4 的 PDT 如何调节线粒体活性和细胞因子反应，揭示了细胞生存和应激适应所必需的细胞途径。这项工作增强了我们对 PDT 治疗乳腺癌的潜力和机制的理解。© 2024。作者获得 Springer-Verlag London Ltd.（Springer Nature 旗下公司）的独家许可。

In this study, we assess the impact of photodynamic therapy (PDT) using aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability and cellular stress responses of MCF-7 breast cancer cells. Specifically, we investigate changes in cell viability, cytokine production, and the expression of stress-related genes. Experimental groups included control cells, those treated with AlPcS4 only, light-emitting diode (LED) only, and combined PDT. To evaluate these effects on cell viability, cytokine production, and the expression of stress-related genes, techniques such as 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, enzyme-linked immunosorbent assays (ELISA), and real-time quantitative PCR (RT‒qPCR) were employed. Our findings reveal how PDT with AlPcS4 modulates mitochondrial activity and cytokine responses, shedding light on the cellular pathways essential for cell survival and stress adaptation. This work enhances our understanding of PDT's therapeutic potential and mechanisms in treating breast cancer.© 2024. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.